Translational regulation of infC operon in Bacillus stearothermophilus - PubMed

Affiliations

• PMID: 8722023
• DOI: 10.1139/o95-115

Review

Translational regulation of infC operon in Bacillus stearothermophilus

D Pediconi et al. Biochem Cell Biol. 1995 Nov-Dec.

Abstract

A Bacillus stearothermophilus in vitro translational system has been developed to study the expression of the three cistrons (infC, rpml, and rplT) constituting the infC operon of this bacterium. When directed by homologous in vitro transcribed infC tricistronic mRNA, this system, which consists of partially purified and purified components of the B. stearothermophilus translational apparatus, synthesizes with high efficiency and specificity the three gene products (IF3, L35, and L20) in a ratio similar to that found in vivo (i.e., about 1:6:6). The three cistrons are translationally coupled and expressed in a specific temporal order: a low level of IF3 synthesis stimulates the expression of L35 which, in turn, greatly stimulates the synthesis of L20 and IF3. Protein L20 and an excess of IF3 were found to act as translational feedback inhibitors of the entire operon. The synthesis of IF3 displayed a strong dependence on IF2. This dependence as well as the repressibility by excess IF3 were found to be due to the presence of the rare AUU initiation triplet at the beginning of infC.

Similar articles

• In vivo transcriptional pattern in the infC operon of Bacillus stearothermophilus.

  Falconi M, Brombach M, Gualerzi CO, Pon CL. Falconi M, et al. Mol Gen Genet. 1991 May;227(1):60-4. doi: 10.1007/BF00260707. Mol Gen Genet. 1991. PMID: 2046659

• Translated translational operator in Escherichia coli. Auto-regulation in the infC-rpmI-rplT operon.

  Lesage P, Truong HN, Graffe M, Dondon J, Springer M. Lesage P, et al. J Mol Biol. 1990 Jun 5;213(3):465-75. doi: 10.1016/S0022-2836(05)80208-6. J Mol Biol. 1990. PMID: 2191140

• Messenger RNA secondary structure and translational coupling in the Escherichia coli operon encoding translation initiation factor IF3 and the ribosomal proteins, L35 and L20.

  Lesage P, Chiaruttini C, Graffe M, Dondon J, Milet M, Springer M. Lesage P, et al. J Mol Biol. 1992 Nov 20;228(2):366-86. doi: 10.1016/0022-2836(92)90827-7. J Mol Biol. 1992. PMID: 1453449

• Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT.

  Pon CL, Brombach M, Thamm S, Gualerzi CO. Pon CL, et al. Mol Gen Genet. 1989 Aug;218(2):355-7. doi: 10.1007/BF00331290. Mol Gen Genet. 1989. PMID: 2779520

• Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation in Escherichia coli.

  Olsson CL, Graffe M, Springer M, Hershey JW. Olsson CL, et al. Mol Gen Genet. 1996 Apr 10;250(6):705-14. doi: 10.1007/BF02172982. Mol Gen Genet. 1996. PMID: 8628231

Cited by

• In polycistronic Qbeta RNA, single-strandedness at one ribosome binding site directly affects translational initiations at a distal upstream cistron.

  Jayant L, Priano C, Mills DR. Jayant L, et al. Nucleic Acids Res. 2010 Nov;38(20):7199-210. doi: 10.1093/nar/gkq541. Epub 2010 Jun 25. Nucleic Acids Res. 2010. PMID: 20581118 Free PMC article.

• Selective stimulation of translation of leaderless mRNA by initiation factor 2: evolutionary implications for translation.

  Grill S, Gualerzi CO, Londei P, Bläsi U. Grill S, et al. EMBO J. 2000 Aug 1;19(15):4101-10. doi: 10.1093/emboj/19.15.4101. EMBO J. 2000. PMID: 10921890 Free PMC article.

• UID-Dual Transcriptome Sequencing Analysis of the Molecular Interactions between Streptococcus agalactiae ATCC 27956 and Mammary Epithelial Cells.

  Gong J, Li T, Li Y, Xiong X, Xu J, Chai X, Ma Y. Gong J, et al. Animals (Basel). 2024 Sep 5;14(17):2587. doi: 10.3390/ani14172587. Animals (Basel). 2024. PMID: 39272372 Free PMC article.

• Cold-stress-induced de novo expression of infC and role of IF3 in cold-shock translational bias.

  Giuliodori AM, Brandi A, Giangrossi M, Gualerzi CO, Pon CL. Giuliodori AM, et al. RNA. 2007 Aug;13(8):1355-65. doi: 10.1261/rna.455607. Epub 2007 Jun 25. RNA. 2007. PMID: 17592046 Free PMC article.

• Proteomic profiling spotlights the molecular targets and the impact of the natural antivirulent umbelliferone on stress response, virulence factors, and the quorum sensing network of Pseudomonas aeruginosa.

  Kasthuri T, Barath S, Nandhakumar M, Karutha Pandian S. Kasthuri T, et al. Front Cell Infect Microbiol. 2022 Nov 30;12:998540. doi: 10.3389/fcimb.2022.998540. eCollection 2022. Front Cell Infect Microbiol. 2022. PMID: 36530435 Free PMC article.

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Atypon

• Molecular Biology Databases

  • BioCyc