Mechanisms of transcriptional activation and repression can both involve TFIID - PubMed

Affiliations

• PMID: 8735274
• DOI: 10.1098/rstb.1996.0050

Review

Mechanisms of transcriptional activation and repression can both involve TFIID

J L Manley et al. Philos Trans R Soc Lond B Biol Sci. 1996.

Abstract

Regulation of transcription involves the activities of activators and repressors. Recent experiments have provided evidence that the function of both types of regulators can involve interactions with one or more component of the basal transcription machinery. A principal target appears to be TFIID, which consists of the TATA binding protein (TBP) and associated factors (TAFs). Here we describe experiments that provide added support for the idea that interactions affecting TFIID can play important roles in both activation and repression. We show, using transfection assays in Drosophila Schneider cells, that recruitment of TBP to a promoter as a GAL4-TBP fusion protein can provide a substantial activation of transcription. The conserved core of TBP is necessary and sufficient for this effect, which was observed with both TATA-containing and TATA-lacking basal promoters. These findings extend experiments performed in yeast, and strengthen the idea that recruitment of TBP (TFIID) can be an important mechanism of activation. We also provide further support for the idea that TBP can be a target for a transcriptional repressor, the Drosophila Even-skipped protein (Eve). We present evidence that the homeodomain, which is necessary for binding TBP in vitro, can also be required for repression in vivo, independent of its role in DNA binding. On the other hand, deletion of the alanine/proline-rich region that is essential for repression in vivo and TBP binding in vitro does not significantly affect DNA binding by the purified protein. These results strengthen the view that TBP, either directly or indirectly as a component of TFIID, can be a target of both activators and repressors.

Similar articles

• Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme.

  Kuras L, Struhl K. Kuras L, et al. Nature. 1999 Jun 10;399(6736):609-13. doi: 10.1038/21239. Nature. 1999. PMID: 10376605

• TBP-associated factors are not generally required for transcriptional activation in yeast.

  Moqtaderi Z, Bai Y, Poon D, Weil PA, Struhl K. Moqtaderi Z, et al. Nature. 1996 Sep 12;383(6596):188-91. doi: 10.1038/383188a0. Nature. 1996. PMID: 8774887

• Even-skipped represses transcription by binding TATA binding protein and blocking the TFIID-TATA box interaction.

  Li C, Manley JL. Li C, et al. Mol Cell Biol. 1998 Jul;18(7):3771-81. doi: 10.1128/MCB.18.7.3771. Mol Cell Biol. 1998. PMID: 9632760 Free PMC article.

• Retinoid-dependent transcription: the RAR/RXR-TBP-EIA/EIA-LA connection.

  Meyer M, Sonntag-Buck V, Keaveney M, Stunnenberg HG. Meyer M, et al. Biochem Soc Symp. 1996;62:97-109. Biochem Soc Symp. 1996. PMID: 8971343 Review.

• TAFs mediate transcriptional activation and promoter selectivity.

  Verrijzer CP, Tjian R. Verrijzer CP, et al. Trends Biochem Sci. 1996 Sep;21(9):338-42. Trends Biochem Sci. 1996. PMID: 8870497 Review.

Cited by

• Evolutionary conserved mechanism of transcriptional repression by even-skipped.

  McKay LM, Carpenter B, Roberts SG. McKay LM, et al. Nucleic Acids Res. 1999 Aug 1;27(15):3064-70. doi: 10.1093/nar/27.15.3064. Nucleic Acids Res. 1999. PMID: 10454601 Free PMC article.

• Heterozygous disruption of the TATA-binding protein gene in DT40 cells causes reduced cdc25B phosphatase expression and delayed mitosis.

  Um M, Yamauchi J, Kato S, Manley JL. Um M, et al. Mol Cell Biol. 2001 Apr;21(7):2435-48. doi: 10.1128/MCB.21.7.2435-2448.2001. Mol Cell Biol. 2001. PMID: 11259592 Free PMC article.

• Groucho augments the repression of multiple Even skipped target genes in establishing parasegment boundaries.

  Kobayashi M, Goldstein RE, Fujioka M, Paroush Z, Jaynes JB. Kobayashi M, et al. Development. 2001 May;128(10):1805-15. doi: 10.1242/dev.128.10.1805. Development. 2001. PMID: 11311161 Free PMC article.

• Trimeric complexes of Antp-TBP with TFIIEβ or Exd modulate transcriptional activity.

  Jiménez-Mejía G, Montalvo-Méndez R, Hernández-Bautista C, Altamirano-Torres C, Vázquez M, Zurita M, Reséndez-Pérez D. Jiménez-Mejía G, et al. Hereditas. 2022 May 30;159(1):23. doi: 10.1186/s41065-022-00239-8. Hereditas. 2022. PMID: 35637493 Free PMC article.

• The Drosophila TATA binding protein contains a strong but masked activation domain.

  Um M, Manley JL. Um M, et al. Gene Expr. 2000;9(3):123-32. doi: 10.3727/000000001783992669. Gene Expr. 2000. PMID: 11243409 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Molecular Biology Databases

  • FlyBase