Partial purification and characterization of an esterase from Fusarium sporotrichioides - PubMed

Partial purification and characterization of an esterase from Fusarium sporotrichioides

J J Park et al. Nat Toxins. 1996.

Abstract

Kinetics analysis of the growth of Fusarium sporotrichioides T-424 at 15 degrees C and 25 degrees C in liquid culture for 35 days revealed that production of deacetylated trichothecenes was associated with an increased activity in fungal esterases. High temperature (25 degrees C) favored enzyme production and enhanced esterase activity. Electrophoresis of crude extracts from the mycelia of F. sporotrichioides T-424 with carboxylesterase staining revealed that several esterases were produced by the fungus. Four carboxylesterase isoenzymes (I-IV) were separated on a DEAE-Sephadex anion exchange column. Type (III) esterase, having activities with the substrate 4-nitrophenylacetate and acetanilide, as well as hydrolytic activity for T-2 toxin and acetyl-T-2 toxin, was partially purified with ammonium sulfate precipitation, immunoaffinity column chromatography, and DEAE-Sephadex A-50 chromatography. The esterase (III) had a molecular weight around 68 kDa in SDS-PAGE. For the deacylation of T-2 toxin and acetyl-T-2 toxin, type (III) esterase had a high specificity for the acetyl group at the C-3 and C-4 positions. The Km values for acetyl-T-2 and T-2 toxin were found to be 41.35 microM and 0.38 microM, respectively. The Km value for the acetyl group at C-3 is 110 times greater than for that at C-4.

Similar articles

• Purification and characterization of an esterase involved in cellulose acetate degradation by Neisseria sicca SB.

  Moriyoshi K, Ohmoto T, Ohe T, Sakai K. Moriyoshi K, et al. Biosci Biotechnol Biochem. 1999 Oct;63(10):1708-13. doi: 10.1271/bbb.63.1708. Biosci Biotechnol Biochem. 1999. PMID: 10586499

• Purification and characterization of two rat liver microsomal carboxylesterases (hydrolase A and B).

  Morgan EW, Yan B, Greenway D, Petersen DR, Parkinson A. Morgan EW, et al. Arch Biochem Biophys. 1994 Dec;315(2):495-512. doi: 10.1006/abbi.1994.1531. Arch Biochem Biophys. 1994. PMID: 7986098

• Purification and characterization of a thermostable carboxylesterase from the thermoacidophilic eubacterium Bacillus acidocaldarius.

  Manco G, Di Gennaro S, De Rosa M, Rossi M. Manco G, et al. Eur J Biochem. 1994 May 1;221(3):965-72. doi: 10.1111/j.1432-1033.1994.tb18812.x. Eur J Biochem. 1994. PMID: 8181479

• Isolation and properties of nonspecific esterase of murine L 1210 leukemia cells.

  Leszczyński D, Kawiak J. Leszczyński D, et al. Acta Histochem Suppl. 1984;30:137-44. Acta Histochem Suppl. 1984. PMID: 6425935

• Molecular aspects of carboxylesterase isoforms in comparison with other esterases.

  Satoh T, Hosokawa M. Satoh T, et al. Toxicol Lett. 1995 Dec;82-83:439-45. doi: 10.1016/0378-4274(95)03493-5. Toxicol Lett. 1995. PMID: 8597091 Review.

Cited by

• Accumulation kinetics of three scirpentriol-based toxins in oats inoculated in Vitro with isolates of Fusarium sporotrichioides and Fusarium poae.

  Schollenberger M, Müller HM, Liebscher M, Schlecker C, Berger M, Hermann W. Schollenberger M, et al. Toxins (Basel). 2011 May;3(5):442-52. doi: 10.3390/toxins3050442. Epub 2011 May 9. Toxins (Basel). 2011. PMID: 22069718 Free PMC article.

• Fusarium toxins of the scirpentriol subgroup: a review.

  Schollenberger M, Drochner W, Müller HM. Schollenberger M, et al. Mycopathologia. 2007 Sep;164(3):101-18. doi: 10.1007/s11046-007-9036-5. Epub 2007 Jul 3. Mycopathologia. 2007. PMID: 17610049 Review.

• Fusarium Tri8 encodes a trichothecene C-3 esterase.

  McCormick SP, Alexander NJ. McCormick SP, et al. Appl Environ Microbiol. 2002 Jun;68(6):2959-64. doi: 10.1128/AEM.68.6.2959-2964.2002. Appl Environ Microbiol. 2002. PMID: 12039755 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Miscellaneous

  • NCI CPTAC Assay Portal