Diversity and specialization of mammalian SWI/SNF complexes - PubMed

Affiliations

• PMID: 8804307
• DOI: 10.1101/gad.10.17.2117

Free article

Comparative Study

Diversity and specialization of mammalian SWI/SNF complexes

W Wang et al. Genes Dev. 1996.

Free article

Abstract

The SWI/SNF complex in yeast facilitates the function of transcriptional activators by opposing chromatin-dependent repression of transcription. We demonstrate that in mammals SWI/SNF complexes are present in multiple forms made up of 9-12 proteins that we refer to as BRG1-associated factors (BAFs) ranging from 47 to 250 kD. We have isolated cDNAs for human BAF155, BAF170, and BAF60. BAF155 and BAF170 are encoded by separate genes that are both homologs of yeast SWI3. Both contain a region of similarity to the DNA binding domain of myb, but lack the basic residues known to be necessary for interaction with DNA. The two SWI3 homologs copurify on antibody columns specific for either BAF155 or BAF170, indicating that they are in the same complex. BAF60 is encoded by a novel gene family. An open reading frame from yeast, which is highly homologous, encodes the previously uncharacterized 73-kD subunit of the yeast SWI/SNF complex required for transcriptional activation by the glucocorticoid receptor (Cairns et al., this issue). BAF60a is expressed in all tissues examined, whereas BAF60b and BAF60c are expressed preferentially in muscle and pancreas, respectively. BAF60a is present within the 2000-kD BRG1 complex, whereas BAF60b is in a distinct complex that shares some but not all subunits with the BRG1 complex. The observed similarity between mammalian BAF190, BAF170, BAF155, BAF60, and BAF47 and yeast SNF2/SWI2, SWI3, SWI3, SWP73, and SNF5, respectively, underscores the similarity of the mammalian and yeast complexes. However, the complexes in mammals are more diverse than the SWI/SNF complex in yeast and are likely dedicated to developmentally distinct functions.

Similar articles

• Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae.

  Treich I, Carlson M. Treich I, et al. Mol Cell Biol. 1997 Apr;17(4):1768-75. doi: 10.1128/MCB.17.4.1768. Mol Cell Biol. 1997. PMID: 9121424 Free PMC article.

• AtSWI3B, an Arabidopsis homolog of SWI3, a core subunit of yeast Swi/Snf chromatin remodeling complex, interacts with FCA, a regulator of flowering time.

  Sarnowski TJ, Swiezewski S, Pawlikowska K, Kaczanowski S, Jerzmanowski A. Sarnowski TJ, et al. Nucleic Acids Res. 2002 Aug 1;30(15):3412-21. doi: 10.1093/nar/gkf458. Nucleic Acids Res. 2002. PMID: 12140326 Free PMC article.

• Two human homologues of Saccharomyces cerevisiae SWI2/SNF2 and Drosophila brahma are transcriptional coactivators cooperating with the estrogen receptor and the retinoic acid receptor.

  Chiba H, Muramatsu M, Nomoto A, Kato H. Chiba H, et al. Nucleic Acids Res. 1994 May 25;22(10):1815-20. doi: 10.1093/nar/22.10.1815. Nucleic Acids Res. 1994. PMID: 8208605 Free PMC article.

• The mammalian SWI/SNF complex and the control of cell growth.

  Muchardt C, Yaniv M. Muchardt C, et al. Semin Cell Dev Biol. 1999 Apr;10(2):189-95. doi: 10.1006/scdb.1999.0300. Semin Cell Dev Biol. 1999. PMID: 10441072 Review.

• The SWI/SNF complex and cancer.

  Reisman D, Glaros S, Thompson EA. Reisman D, et al. Oncogene. 2009 Apr 9;28(14):1653-68. doi: 10.1038/onc.2009.4. Epub 2009 Feb 23. Oncogene. 2009. PMID: 19234488 Review.

Cited by

• Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture.

  Zhao M, Yang S, Chen CY, Li C, Shan W, Lu W, Cui Y, Liu X, Wu K. Zhao M, et al. PLoS Genet. 2015 Mar 30;11(3):e1005125. doi: 10.1371/journal.pgen.1005125. eCollection 2015 Mar. PLoS Genet. 2015. PMID: 25822547 Free PMC article.

• Rhabdoid tumors: an initial clue to the role of chromatin remodeling in cancer.

  Lee RS, Roberts CW. Lee RS, et al. Brain Pathol. 2013 Mar;23(2):200-5. doi: 10.1111/bpa.12021. Brain Pathol. 2013. PMID: 23432645 Free PMC article. Review.

• Epigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres.

  Albini S, Coutinho P, Malecova B, Giordani L, Savchenko A, Forcales SV, Puri PL. Albini S, et al. Cell Rep. 2013 Mar 28;3(3):661-70. doi: 10.1016/j.celrep.2013.02.012. Epub 2013 Mar 7. Cell Rep. 2013. PMID: 23478022 Free PMC article.

• MBD2 is a critical component of a methyl cytosine-binding protein complex isolated from primary erythroid cells.

  Kransdorf EP, Wang SZ, Zhu SZ, Langston TB, Rupon JW, Ginder GD. Kransdorf EP, et al. Blood. 2006 Oct 15;108(8):2836-45. doi: 10.1182/blood-2006-04-016394. Epub 2006 Jun 15. Blood. 2006. PMID: 16778143 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • HighWire

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource
  • Mouse Genome Informatics (MGI)
  • Saccharomyces Genome Database

• Research Materials

  • Addgene Non-profit plasmid repository

• Miscellaneous

  • NCI CPTAC Assay Portal