Purification and biochemical heterogeneity of the mammalian SWI-SNF complex - PubMed
- ️Mon Jan 01 1996
. 1996 Oct 1;15(19):5370-82.
J Côté, Y Xue, S Zhou, P A Khavari, S R Biggar, C Muchardt, G V Kalpana, S P Goff, M Yaniv, J L Workman, G R Crabtree
Affiliations
- PMID: 8895581
- PMCID: PMC452280
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex
W Wang et al. EMBO J. 1996.
Abstract
We have purified distinct complexes of nine to 12 proteins [referred to as BRG1-associated factors (BAFs)] from several mammalian cell lines using an antibody to the SWI2-SNF2 homolog BRG1. Microsequencing revealed that the 47 kDa BAF is identical to INI1. Previously INI1 has been shown to interact with and activate human immunodeficiency virus integrase and to be homologous to the yeast SNF5 gene. A group of BAF47-associated proteins were affinity purified with antibodies against INI1/BAF47 and were found to be identical to those co-purified with BRG1, strongly indicating that this group of proteins associates tightly and is likely to be the mammalian equivalent of the yeast SWI-SNF complex. Complexes containing BRG1 can disrupt nucleosomes and facilitate the binding of GAL4-VP16 to a nucleosomal template similar to the yeast SWI-SNF complex. Purification of the complex from several cell lines demonstrates that it is heterogeneous with respect to subunit composition. The two SWI-SNF2 homologs, BRG1 and hbrm, were found in separate complexes. Certain cell lines completely lack BRG1 and hbrm, indicating that they are not essential for cell viability and that the mammalian SWI-SNF complex may be tailored to the needs of a differentiated cell type.
Similar articles
-
Doan DN, Veal TM, Yan Z, Wang W, Jones SN, Imbalzano AN. Doan DN, et al. Oncogene. 2004 Apr 22;23(19):3462-73. doi: 10.1038/sj.onc.1207472. Oncogene. 2004. PMID: 14990991
-
Diversity and specialization of mammalian SWI/SNF complexes.
Wang W, Xue Y, Zhou S, Kuo A, Cairns BR, Crabtree GR. Wang W, et al. Genes Dev. 1996 Sep 1;10(17):2117-30. doi: 10.1101/gad.10.17.2117. Genes Dev. 1996. PMID: 8804307
-
Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes.
Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Sif S, et al. Genes Dev. 2001 Mar 1;15(5):603-18. doi: 10.1101/gad.872801. Genes Dev. 2001. PMID: 11238380 Free PMC article.
-
The SWI/SNF complex and cancer.
Reisman D, Glaros S, Thompson EA. Reisman D, et al. Oncogene. 2009 Apr 9;28(14):1653-68. doi: 10.1038/onc.2009.4. Epub 2009 Feb 23. Oncogene. 2009. PMID: 19234488 Review.
-
BAFfling pathologies: Alterations of BAF complexes in cancer.
Arnaud O, Le Loarer F, Tirode F. Arnaud O, et al. Cancer Lett. 2018 Apr 10;419:266-279. doi: 10.1016/j.canlet.2018.01.046. Epub 2018 Jan 31. Cancer Lett. 2018. PMID: 29374542 Review.
Cited by
-
Update from the 2011 International Schwannomatosis Workshop: From genetics to diagnostic criteria.
Plotkin SR, Blakeley JO, Evans DG, Hanemann CO, Hulsebos TJ, Hunter-Schaedle K, Kalpana GV, Korf B, Messiaen L, Papi L, Ratner N, Sherman LS, Smith MJ, Stemmer-Rachamimov AO, Vitte J, Giovannini M. Plotkin SR, et al. Am J Med Genet A. 2013 Mar;161A(3):405-16. doi: 10.1002/ajmg.a.35760. Epub 2013 Feb 7. Am J Med Genet A. 2013. PMID: 23401320 Free PMC article.
-
E2F6 associates with BRG1 in transcriptional regulation.
Leung JY, Nevins JR. Leung JY, et al. PLoS One. 2012;7(10):e47967. doi: 10.1371/journal.pone.0047967. Epub 2012 Oct 17. PLoS One. 2012. PMID: 23082233 Free PMC article.
-
The roles of SNF2/SWI2 nucleosome remodeling enzymes in blood cell differentiation and leukemia.
Prasad P, Lennartsson A, Ekwall K. Prasad P, et al. Biomed Res Int. 2015;2015:347571. doi: 10.1155/2015/347571. Epub 2015 Feb 19. Biomed Res Int. 2015. PMID: 25789315 Free PMC article. Review.
-
Hijacking the chromatin remodeling machinery: impact of SWI/SNF perturbations in cancer.
Weissman B, Knudsen KE. Weissman B, et al. Cancer Res. 2009 Nov 1;69(21):8223-30. doi: 10.1158/0008-5472.CAN-09-2166. Epub 2009 Oct 20. Cancer Res. 2009. PMID: 19843852 Free PMC article. Review.
-
de la Serna IL, Ohkawa Y, Berkes CA, Bergstrom DA, Dacwag CS, Tapscott SJ, Imbalzano AN. de la Serna IL, et al. Mol Cell Biol. 2005 May;25(10):3997-4009. doi: 10.1128/MCB.25.10.3997-4009.2005. Mol Cell Biol. 2005. PMID: 15870273 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous