Metabotropic glutamate receptor 5 is a disulfide-linked dimer - PubMed

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• PMID: 8910492
• DOI: 10.1074/jbc.271.45.28612

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Metabotropic glutamate receptor 5 is a disulfide-linked dimer

C Romano et al. J Biol Chem. 1996.

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Abstract

The sequences of the metabotropic glutamate receptors (mGluRs) show little homology with other members of the G protein-coupled receptor family and exhibit several distinctive features, including a large N-terminal extracellular domain with 17 cysteines in conserved positions. Here we demonstrate that mGluR5, as well as other mGluRs, behave as species approximately twice as large as expected from their sequence, but reducing conditions cause a decrease to the predicted molecular mass. Co-immunoprecipitation experiments using wild type and epitope-tagged receptors demonstrate that this is due to specific, disulfide-dependent dimerization of the receptor. The intermolecular disulfide that mediates dimerization occurs in the extracellular domain, within about 17 kDa from the N terminus.

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