A new tinman-related gene, nkx2.7, anticipates the expression of nkx2.5 and nkx2.3 in zebrafish heart and pharyngeal endoderm - PubMed

Affiliations

• PMID: 8954740
• DOI: 10.1006/dbio.1996.0341

Free article

Comparative Study

A new tinman-related gene, nkx2.7, anticipates the expression of nkx2.5 and nkx2.3 in zebrafish heart and pharyngeal endoderm

K H Lee et al. Dev Biol. 1996.

Free article

Abstract

The Drosophila homeobox gene tinman and its vertebrate homologs Nkx-2.5 and Nkx-2.3 are critical determinants of cardiac development. We report here the identification of a new tinman-related gene, nkx2.7, as well as orthologs of Nkx-2.5 and Nkx-2.3 in the zebrafish. Analysis of their expression in the developing zebrafish embryo reveals that nkx2.7 transcripts are the first to appear in cardiac mesodermal and pharyngeal endodermal precursors of the anterior hypoblast, anticipating both temporally and spatially the later expression of nkx2.5 and nkx2.3 in these lineages. The preeminence of nkx2.7 in these embryonic lineages is consistent with a key role in cell fate determination, perhaps in part through the induction of nkx2.5 and nkx2.3. The findings provide the first molecular clues as to the spatial organization of endodermal and cardiac mesodermal precursors in the zebrafish hypoblast immediately following gastrulation. They suggest a coordinate role for these three tinman-related genes in the development of the heart and pharyngeal arches, and reinforce the paradigm of gene duplication and subspecialization between Drosophila and vertebrate species. The results provide a framework in which to analyze potential changes in tinman-related gene expression during abnormal zebrafish development.

Similar articles

• Chicken Nkx-2.8: a novel homeobox gene expressed in early heart progenitor cells and pharyngeal pouch-2 and -3 endoderm.

  Reecy JM, Yamada M, Cummings K, Sosic D, Chen CY, Eichele G, Olson EN, Schwartz RJ. Reecy JM, et al. Dev Biol. 1997 Aug 15;188(2):295-311. doi: 10.1006/dbio.1997.8641. Dev Biol. 1997. PMID: 9268576

• Nkx2.5 and Nkx2.6, homologs of Drosophila tinman, are required for development of the pharynx.

  Tanaka M, Schinke M, Liao HS, Yamasaki N, Izumo S. Tanaka M, et al. Mol Cell Biol. 2001 Jul;21(13):4391-8. doi: 10.1128/MCB.21.13.4391-4398.2001. Mol Cell Biol. 2001. PMID: 11390666 Free PMC article.

• Vertebrate homologs of tinman and bagpipe: roles of the homeobox genes in cardiovascular development.

  Tanaka M, Kasahara H, Bartunkova S, Schinke M, Komuro I, Inagaki H, Lee Y, Lyons GE, Izumo S. Tanaka M, et al. Dev Genet. 1998;22(3):239-49. doi: 10.1002/(SICI)1520-6408(1998)22:3<239::AID-DVG6>3.0.CO;2-7. Dev Genet. 1998. PMID: 9621431

• Vertebrate tinman homologues and cardiac differentiation.

  Evans SM. Evans SM. Semin Cell Dev Biol. 1999 Feb;10(1):73-83. doi: 10.1006/scdb.1999.0282. Semin Cell Dev Biol. 1999. PMID: 10355031 Review.

• Developmental paradigms in heart disease: insights from tinman.

  Prall OW, Elliott DA, Harvey RP. Prall OW, et al. Ann Med. 2002;34(3):148-56. Ann Med. 2002. PMID: 12173684 Review.

Cited by

• Fscn1 is required for the trafficking of TGF-β family type I receptors during endoderm formation.

  Liu Z, Ning G, Xu R, Cao Y, Meng A, Wang Q. Liu Z, et al. Nat Commun. 2016 Aug 22;7:12603. doi: 10.1038/ncomms12603. Nat Commun. 2016. PMID: 27545838 Free PMC article.

• Nkx genes establish second heart field cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 repression.

  Colombo S, de Sena-Tomás C, George V, Werdich AA, Kapur S, MacRae CA, Targoff KL. Colombo S, et al. Development. 2018 Feb 5;145(3):dev161497. doi: 10.1242/dev.161497. Development. 2018. PMID: 29361575 Free PMC article.

• Unique developmental trajectories and genetic regulation of ventricular and outflow tract progenitors in the zebrafish second heart field.

  Paffett-Lugassy N, Novikov N, Jeffrey S, Abrial M, Guner-Ataman B, Sakthivel S, Burns CE, Burns CG. Paffett-Lugassy N, et al. Development. 2017 Dec 15;144(24):4616-4624. doi: 10.1242/dev.153411. Epub 2017 Oct 23. Development. 2017. PMID: 29061637 Free PMC article.

• Tbl3 regulates cell cycle length during zebrafish development.

  Hutchinson SA, Tooke-Locke E, Wang J, Tsai S, Katz T, Trede NS. Hutchinson SA, et al. Dev Biol. 2012 Aug 15;368(2):261-72. doi: 10.1016/j.ydbio.2012.05.024. Epub 2012 May 30. Dev Biol. 2012. PMID: 22659140 Free PMC article.

• Wnt-dependent epithelial transitions drive pharyngeal pouch formation.

  Choe CP, Collazo A, Trinh le A, Pan L, Moens CB, Crump JG. Choe CP, et al. Dev Cell. 2013 Feb 11;24(3):296-309. doi: 10.1016/j.devcel.2012.12.003. Epub 2013 Jan 31. Dev Cell. 2013. PMID: 23375584 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Molecular Biology Databases

  • ZFIN

• Research Materials

  • NCI CPTC Antibody Characterization Program