The potential of extrachromosomal replicating vectors for gene therapy - PubMed

Review

The potential of extrachromosomal replicating vectors for gene therapy

M P Calos. Trends Genet. 1996 Nov.

Free article

Abstract

Persistence of DNA vectors in target cells is advantageous in most applications of gene therapy. Particularly when target cells are undergoing proliferation, vector longevity will depend on either the integration of the vector into the chromosomes or the ability of the vector to replicate and be retained extrachromosomally. Vectors that efficiently integrate in a nonrandom fashion are currently unavailable, and those that can replicate extrachromosomally provide a major alternative strategy. Several classes of such vectors are under development, carrying mechanisms for prolonging DNA retention in mammalian nuclei that extend vector lifetime in non-proliferating cells as well. The vectors utilize either chromosomal or viral elements to mediate replication and retention, and have a large size capacity for insertion of genes of interest. I discuss the state of the art for these vectors, including the assets and limitations of their future use in gene therapy.

Similar articles

• Mammalian artificial chromosomes--vectors for somatic gene therapy.

  Ascenzioni F, Donini P, Lipps HJ. Ascenzioni F, et al. Cancer Lett. 1997 Oct 14;118(2):135-42. doi: 10.1016/s0304-3835(97)00323-6. Cancer Lett. 1997. PMID: 9459203 Review.

• The functional role of S/MARs in episomal vectors as defined by the stress-induced destabilization profile of the vector sequences.

  Giannakopoulos A, Stavrou EF, Zarkadis I, Zoumbos N, Thrasher AJ, Athanassiadou A. Giannakopoulos A, et al. J Mol Biol. 2009 Apr 17;387(5):1239-49. doi: 10.1016/j.jmb.2009.02.043. Epub 2009 Feb 25. J Mol Biol. 2009. PMID: 19248788

• Long-term gene expression from autonomously replicating vectors in mammalian cells.

  Wohlgemuth JG, Kang SH, Bulboaca GH, Nawotka KA, Calos MP. Wohlgemuth JG, et al. Gene Ther. 1996 Jun;3(6):503-12. Gene Ther. 1996. PMID: 8789800

• Analysis of a YAC with human telomeres and oriP from epstein-barr virus in yeast and 293 cells.

  Tolmachova T, Simpson K, Huxley C. Tolmachova T, et al. Nucleic Acids Res. 1999 Sep 15;27(18):3736-44. doi: 10.1093/nar/27.18.3736. Nucleic Acids Res. 1999. PMID: 10471744 Free PMC article.

• Episomal vectors for gene therapy.

  Ehrhardt A, Haase R, Schepers A, Deutsch MJ, Lipps HJ, Baiker A. Ehrhardt A, et al. Curr Gene Ther. 2008 Jun;8(3):147-61. doi: 10.2174/156652308784746440. Curr Gene Ther. 2008. PMID: 18537590 Review.

Cited by

• "Alternative" endocytic mechanisms exploited by pathogens: new avenues for therapeutic delivery?

  Medina-Kauwe LK. Medina-Kauwe LK. Adv Drug Deliv Rev. 2007 Aug 10;59(8):798-809. doi: 10.1016/j.addr.2007.06.009. Epub 2007 Jul 5. Adv Drug Deliv Rev. 2007. PMID: 17707545 Free PMC article. Review.

• Untangling the relationships between DNA repair pathways by silencing more than 20 DNA repair genes in human stable clones.

  Biard DS. Biard DS. Nucleic Acids Res. 2007;35(11):3535-50. doi: 10.1093/nar/gkm195. Epub 2007 May 5. Nucleic Acids Res. 2007. PMID: 17483520 Free PMC article.

• Bovine papillomavirus type 1 genomes and the E2 transactivator protein are closely associated with mitotic chromatin.

  Skiadopoulos MH, McBride AA. Skiadopoulos MH, et al. J Virol. 1998 Mar;72(3):2079-88. doi: 10.1128/JVI.72.3.2079-2088.1998. J Virol. 1998. PMID: 9499063 Free PMC article.

• Stability without a centromere.

  Calos MP. Calos MP. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4084-5. doi: 10.1073/pnas.95.8.4084. Proc Natl Acad Sci U S A. 1998. PMID: 9539691 Free PMC article. Review. No abstract available.

• Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 - new vectors for in vitro and in vivo delivery.

  Magin-Lachmann C, Kotzamanis G, D'Aiuto L, Wagner E, Huxley C. Magin-Lachmann C, et al. BMC Biotechnol. 2003 Feb 3;3(1):2. doi: 10.1186/1472-6750-3-2. BMC Biotechnol. 2003. PMID: 12609052 Free PMC article.

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information