Crystal growth in dental enamel: the role of amelogenins and albumin - PubMed

Crystal growth in dental enamel: the role of amelogenins and albumin

C Robinson et al. Adv Dent Res. 1996 Nov.

Abstract

Amelogenin-mineral interactions were investigated using an in vitro binding approach. Rat incisor enamel matrix proteins (mainly amelogenins) were dissolved in synthetic enamel fluid and allowed to equilibrate with deproteinised developing enamel crystals. The results showed that amlogenin proteins of 21, 23, 24, 26 and 27-kDa (corresponding to nascent and partially degraded amelogenins) were associated with the crystals whilst the lower Mr amelogenins (< 21 KDa) remained free in the synthetic enamel fluid. These data suggest the nascent and partially degraded amelogenins may interact with developing enamel crystals and could influence their growth. Albumin-mineral interactions were investigated by extracting developing rat incisor enamel with synthetic enamel fluid. Insoluble material (including the enamel crystals) was then further extracted with 0.1 M phosphate buffer (pH 7.4) to desorb any mineral bound proteins. Western blotting using anti-albumin antibodies showed that almost all of the albumin from the secretory stage enamel and a significant proportion of the albumin present in early transition stage was extractable in the synthetic enamel fluid. However, synthetic enamel fluid did not extract albumin from late transition or maturation stage tissue, which could only be removed following further extraction with phosphate buffer. Albumin degradation was apparent during the transition and maturation stages, where it is degraded and ultimately removed. This binding pattern may be related to amelogenin degradation and removal during the transition stage, permitting albumin access to the previously obscured crystal surfaces. That the secretory stage matrix appears to "protect" secretory stage crystals from albumin may be an important consideration in the aetiology of enamel hypoplasias (i.e. incomplete crystal growth) and when using dissociative extraction procedures for the identification of mineral bound proteins.

Similar articles

• Amelin extracellular processing and aggregation during rat incisor amelogenesis.

  Brookes SJ, Kirkham J, Shore RC, Wood SR, Slaby I, Robinson C. Brookes SJ, et al. Arch Oral Biol. 2001 Mar;46(3):201-8. doi: 10.1016/s0003-9969(00)00121-7. Arch Oral Biol. 2001. PMID: 11165565

• Recent observations on enamel crystal formation during mammalian amelogenesis.

  Aoba T. Aoba T. Anat Rec. 1996 Jun;245(2):208-18. doi: 10.1002/(SICI)1097-0185(199606)245:2<208::AID-AR8>3.0.CO;2-S. Anat Rec. 1996. PMID: 8769664 Review.

• Uptake and metabolism of albumin by rodent incisor enamel in vivo and postmortem: implications for control of mineralization by albumin.

  Robinson C, Brookes SJ, Kirkham J, Shore RC, Bonass WA. Robinson C, et al. Calcif Tissue Int. 1994 Dec;55(6):467-72. doi: 10.1007/BF00298561. Calcif Tissue Int. 1994. PMID: 7895186

• Evidence for charge domains on developing enamel crystal surfaces.

  Kirkham J, Zhang J, Brookes SJ, Shore RC, Wood SR, Smith DA, Wallwork ML, Ryu OH, Robinson C. Kirkham J, et al. J Dent Res. 2000 Dec;79(12):1943-7. doi: 10.1177/00220345000790120401. J Dent Res. 2000. PMID: 11201043

• Amelogenesis: Transformation of a protein-mineral matrix into tooth enamel.

  Pandya M, Diekwisch TGH. Pandya M, et al. J Struct Biol. 2021 Dec;213(4):107809. doi: 10.1016/j.jsb.2021.107809. Epub 2021 Nov 6. J Struct Biol. 2021. PMID: 34748943 Free PMC article. Review.

Cited by

• Tooth Enamel and its Dynamic Protein Matrix.

  Gil-Bona A, Bidlack FB. Gil-Bona A, et al. Int J Mol Sci. 2020 Jun 23;21(12):4458. doi: 10.3390/ijms21124458. Int J Mol Sci. 2020. PMID: 32585904 Free PMC article. Review.

• Phenotypic Analysis, Molecular Characterization, and Antibiogram of Caries-Causing Bacteria Isolated from Dental Patients.

  Farva K, Sattar H, Ullah H, Raziq A, Mehmood MD, Tareen AK, Sultan IN, Zohra Q, Khan MW. Farva K, et al. Microorganisms. 2023 Jul 31;11(8):1952. doi: 10.3390/microorganisms11081952. Microorganisms. 2023. PMID: 37630520 Free PMC article.

• Evolutionary story of mammalian-specific amelogenin exons 4, "4b", 8, and 9.

  Sire JY, Huang Y, Li W, Delgado S, Goldberg M, Denbesten PK. Sire JY, et al. J Dent Res. 2012 Jan;91(1):84-9. doi: 10.1177/0022034511423399. Epub 2011 Sep 26. J Dent Res. 2012. PMID: 21948850 Free PMC article.

• A N-Terminus Domain Determines Amelogenin's Stability to Guide the Development of Mouse Enamel Matrix.

  Huang Y, Bai Y, Chang C, Bacino M, Cheng IC, Li L, Habelitz S, Li W, Zhang Y. Huang Y, et al. J Bone Miner Res. 2021 Sep;36(9):1781-1795. doi: 10.1002/jbmr.4329. Epub 2021 May 25. J Bone Miner Res. 2021. PMID: 33957008 Free PMC article.

• A Breakthrough in Understanding the Pathogenesis of Molar Hypomineralisation: The Mineralisation-Poisoning Model.

  Hubbard MJ, Mangum JE, Perez VA, Williams R. Hubbard MJ, et al. Front Physiol. 2021 Dec 21;12:802833. doi: 10.3389/fphys.2021.802833. eCollection 2021. Front Physiol. 2021. PMID: 34992550 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon