pubmed.ncbi.nlm.nih.gov

Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins - PubMed

Comparative Study

Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins

L Hammarström et al. J Clin Periodontol. 1997 Sep.

Abstract

There is increasing evidence that cells of the epithelial root sheath synthesize enamel matrix proteins and that these proteins play a fundamental role in the formation of acellular cementum, the key tissue in the development of a functional periodontium. The purpose of the present study was to explore the effect of locally applied enamel matrix and different protein fractions of the matrix on periodontal regeneration in a buccal dehiscence model in monkeys. Buccal, mucoperiosteal flaps were raised from the canine to the 1st molar on each side of the maxilla. The buccal alveolar bone plate, the exposed periodontal ligament and cementum were removed. Various preparations of porcine enamel matrix with or without vehicles were applied before the flaps were repositioned and sutured. After 8 weeks, the healing was evaluated in the light microscope, and morphometric comparisons were made. Application of homogenized enamel matrix or an acidic extract of the matrix containing the hydrophobic, low molecular weight proteins, amelogenins, resulted in an almost complete regeneration of acellular cementum, firmly attached to the dentin and with collagenous fibers extending over to newly formed alveolar bone. After application of fractions obtained by neutral EDTA extraction containing the acidic, high molecular weight proteins of the enamel matrix, very little new cementum was formed and hardly any new bone. The results of the controls in which no test substance was applied before the repositioning of the flap, were very similar to those obtained with the EDTA extracted material. Propylene glycol alginate (PGA), hydroxyethyl cellulose and dextran were tried as vehicles for the enamel matrix preparations. Only PGA in combination with the amelogenin fraction resulted in significant regeneration of the periodontal tissues.

PubMed Disclaimer

Cited by

Enamel matrix protein derivatives: role in periodontal regeneration.
Rathva VJ. Rathva VJ. Clin Cosmet Investig Dent. 2011 Dec 1;3:79-92. doi: 10.2147/CCIDEN.S25347. Print 2011. Clin Cosmet Investig Dent. 2011. PMID: 23674918 Free PMC article.
Analogous effects of recombinant human full-length amelogenin expressed by Pichia pastoris yeast and enamel matrix derivative in vitro.
Cheng L, Lin ZK, Shu R, Liu DL, Zhang XL, Liu B, Wang J, Tian L. Cheng L, et al. Cell Prolif. 2012 Oct;45(5):456-65. doi: 10.1111/j.1365-2184.2012.00834.x. Epub 2012 Jul 26. Cell Prolif. 2012. PMID: 22834823 Free PMC article.
Effects of mineral trioxide aggregate, calcium hydroxide, biodentine and Emdogain on osteogenesis, Odontogenesis, angiogenesis and cell viability of dental pulp stem cells.
Youssef AR, Emara R, Taher MM, Al-Allaf FA, Almalki M, Almasri MA, Siddiqui SS. Youssef AR, et al. BMC Oral Health. 2019 Jul 2;19(1):133. doi: 10.1186/s12903-019-0827-0. BMC Oral Health. 2019. PMID: 31266498 Free PMC article.
Acceleration of bone regeneration of horizontal bone defect in rats using collagen-binding basic fibroblast growth factor combined with collagen scaffolds.
Nakamura S, Ito T, Okamoto K, Mima T, Uchida K, Siddiqui YD, Ito M, Tai M, Okubo K, Yamashiro K, Omori K, Yamamoto T, Matsushita O, Takashiba S. Nakamura S, et al. J Periodontol. 2019 Sep;90(9):1043-1052. doi: 10.1002/JPER.18-0674. Epub 2019 Apr 14. J Periodontol. 2019. PMID: 30889294 Free PMC article.
The Applications of Enamel Matrix Derivative in Implant Dentistry: A Narrative Review.
Alberti A, Francetti L, Taschieri S, Corbella S. Alberti A, et al. Materials (Basel). 2021 Jun 3;14(11):3045. doi: 10.3390/ma14113045. Materials (Basel). 2021. PMID: 34204976 Free PMC article. Review.

Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins - PubMed