Tetracycline-regulated transgene expression in hippocampal neurones following transfection with adenoviral vectors - PubMed

Tetracycline-regulated transgene expression in hippocampal neurones following transfection with adenoviral vectors

T C Harding et al. J Neurochem. 1997 Dec.

Free article

Abstract

A transfer system that enabled the efficient introduction of transgenes into neurones and the quantitative control of the expressed transgene would greatly facilitate studies into neuronal gene function. To develop such a system we incorporated the tetracycline (Tet)-responsive On/Off regulatory elements into type-5 adenoviral (Ad) vectors. Regulation of transgene expression following transfection was measured by placing the enhanced green fluorescent protein (EGFP) gene upstream of the Tet regulatory element. The results showed that cultures of primary hippocampal cells could be transfected with very high efficiency (<70%) by the AdTet-On and AdTet-Off systems. Following transfection with the AdTet-On system no EGFP-fluorescent cells could be detected until doxycycline was added. The AdTet-Off system showed the reverse transcriptional regulation, in that the addition of Tet caused EGFP fluorescence to be abolished.

Similar articles

• Increased utility in the CNS of a powerful neuron-specific tetracycline-regulatable adenoviral system developed using a post-transcriptional enhancer.

  Lee YB, Cosgrave AS, Glover CP, Bienemann A, Heywood D, Hobson RJ, Uney JB. Lee YB, et al. J Gene Med. 2005 May;7(5):576-83. doi: 10.1002/jgm.694. J Gene Med. 2005. PMID: 15580589

• A tetracycline-regulated adenoviral expression system for in vivo delivery of transgenes to lung and liver.

  Tietge UJ, Kozarsky KF, Donahee MH, Rader DJ. Tietge UJ, et al. J Gene Med. 2003 Jul;5(7):567-75. doi: 10.1002/jgm.384. J Gene Med. 2003. PMID: 12825196

• Targeting of tetracycline-regulatable transgene expression specifically to neuronal and glial cell populations using adenoviral vectors.

  Ralph GS, Bienemann A, Harding TC, Hopton M, Henley J, Uney JB. Ralph GS, et al. Neuroreport. 2000 Jun 26;11(9):2051-5. doi: 10.1097/00001756-200006260-00048. Neuroreport. 2000. PMID: 10884069

• Optimizing regulatable gene expression using adenoviral vectors.

  Lee YB, Glover CP, Cosgrave AS, Bienemann A, Uney JB. Lee YB, et al. Exp Physiol. 2005 Jan;90(1):33-7. doi: 10.1113/expphysiol.2004.028209. Epub 2004 Nov 12. Exp Physiol. 2005. PMID: 15542617 Review.

• Improved applications of the tetracycline-regulated gene depletion system.

  Nishijima H, Yasunari T, Nakayama T, Adachi N, Shibahara K. Nishijima H, et al. Biosci Trends. 2009 Oct;3(5):161-7. Biosci Trends. 2009. PMID: 20103842 Review.

Cited by

• Regulatable gene expression systems for gene therapy applications: progress and future challenges.

  Goverdhana S, Puntel M, Xiong W, Zirger JM, Barcia C, Curtin JF, Soffer EB, Mondkar S, King GD, Hu J, Sciascia SA, Candolfi M, Greengold DS, Lowenstein PR, Castro MG. Goverdhana S, et al. Mol Ther. 2005 Aug;12(2):189-211. doi: 10.1016/j.ymthe.2005.03.022. Mol Ther. 2005. PMID: 15946903 Free PMC article. Review.

• Regulatable gene expression systems for gene therapy.

  Vilaboa N, Voellmy R. Vilaboa N, et al. Curr Gene Ther. 2006 Aug;6(4):421-38. doi: 10.2174/156652306777934829. Curr Gene Ther. 2006. PMID: 16918333 Review.

• Conventional protein kinase C and atypical protein kinase Czeta differentially regulate macrophage production of tumour necrosis factor-alpha and interleukin-10.

  Foey AD, Brennan FM. Foey AD, et al. Immunology. 2004 May;112(1):44-53. doi: 10.1111/j.1365-2567.2004.01852.x. Immunology. 2004. PMID: 15096183 Free PMC article.

• Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain.

  Liu B, Wang S, Brenner M, Paton JF, Kasparov S. Liu B, et al. J Gene Med. 2008 May;10(5):583-92. doi: 10.1002/jgm.1178. J Gene Med. 2008. PMID: 18324737 Free PMC article.

• Dual role for mitogen-activated protein kinase (Erk) in insulin-dependent regulation of Fra-1 (fos-related antigen-1) transcription and phosphorylation.

  Hurd TW, Culbert AA, Webster KJ, Tavaré JM. Hurd TW, et al. Biochem J. 2002 Dec 1;368(Pt 2):573-80. doi: 10.1042/BJ20020579. Biochem J. 2002. PMID: 12197835 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database