Dymple, a novel dynamin-like high molecular weight GTPase lacking a proline-rich carboxyl-terminal domain in mammalian cells - PubMed
- ️Thu Jan 01 1998
. 1998 Jan 9;273(2):1044-51.
doi: 10.1074/jbc.273.2.1044.
Affiliations
- PMID: 9422767
- DOI: 10.1074/jbc.273.2.1044
Free article
Dymple, a novel dynamin-like high molecular weight GTPase lacking a proline-rich carboxyl-terminal domain in mammalian cells
T Kamimoto et al. J Biol Chem. 1998.
Free article
Abstract
We have cloned human dymple, a novel dynamin family member. The full-length cDNA sequence encodes a protein composed of 736 amino acids with a molecular mass of 80 kDa. This amino acid sequence most resembles yeast DNM1P and VPS1P. Dymple lacks a proline-rich carboxyl-terminal domain through which dynamin binds to SH3 domains to be activated. Northern blot analysis revealed two transcript sizes of 2.5 and 4.2 kilobases with alternative polyadenylation at the highest levels in brain, skeletal muscle, and testis. It was further established that there are three patterns of alternative splicing producing in-frame deletions in the coding sequence of dymple in a tissue-specific manner. When overexpressed, wild-type dymple exhibited a punctate perinuclear cytoplasmic pattern, whereas an amino-terminal deletion mutant formed large aggregates bounded by a trans-Golgi network marker. Since dynamin participates in clathrin-mediated endocytosis through a well-characterized mechanism, the existence of a dynamin-like molecule in each specific vesicle transport pathway has been predicted. Our findings suggest that dymple may be the first example of such a subfamily in mammalian cells other than dynamin itself, although its precise role and membrane localization remain to be resolved.
Similar articles
-
Imoto M, Tachibana I, Urrutia R. Imoto M, et al. J Cell Sci. 1998 May;111 ( Pt 10):1341-9. doi: 10.1242/jcs.111.10.1341. J Cell Sci. 1998. PMID: 9570752
-
Role of the basic, proline-rich region of dynamin in Src homology 3 domain binding and endocytosis.
Okamoto PM, Herskovits JS, Vallee RB. Okamoto PM, et al. J Biol Chem. 1997 Apr 25;272(17):11629-35. doi: 10.1074/jbc.272.17.11629. J Biol Chem. 1997. PMID: 9111080
-
Grabs D, Slepnev VI, Songyang Z, David C, Lynch M, Cantley LC, De Camilli P. Grabs D, et al. J Biol Chem. 1997 May 16;272(20):13419-25. doi: 10.1074/jbc.272.20.13419. J Biol Chem. 1997. PMID: 9148966
-
The role of the PH domain and SH3 binding domains in dynamin function.
Scaife RM, Margolis RL. Scaife RM, et al. Cell Signal. 1997 Sep;9(6):395-401. doi: 10.1016/s0898-6568(97)00041-7. Cell Signal. 1997. PMID: 9376220 Review.
-
Dynamin and its role in membrane fission.
Hinshaw JE. Hinshaw JE. Annu Rev Cell Dev Biol. 2000;16:483-519. doi: 10.1146/annurev.cellbio.16.1.483. Annu Rev Cell Dev Biol. 2000. PMID: 11031245 Free PMC article. Review.
Cited by
-
Figueroa-Romero C, Iñiguez-Lluhí JA, Stadler J, Chang CR, Arnoult D, Keller PJ, Hong Y, Blackstone C, Feldman EL. Figueroa-Romero C, et al. FASEB J. 2009 Nov;23(11):3917-27. doi: 10.1096/fj.09-136630. Epub 2009 Jul 28. FASEB J. 2009. PMID: 19638400 Free PMC article.
-
Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells.
Smirnova E, Griparic L, Shurland DL, van der Bliek AM. Smirnova E, et al. Mol Biol Cell. 2001 Aug;12(8):2245-56. doi: 10.1091/mbc.12.8.2245. Mol Biol Cell. 2001. PMID: 11514614 Free PMC article.
-
Drp1 mediates caspase-independent type III cell death in normal and leukemic cells.
Bras M, Yuste VJ, Roué G, Barbier S, Sancho P, Virely C, Rubio M, Baudet S, Esquerda JE, Merle-Béral H, Sarfati M, Susin SA. Bras M, et al. Mol Cell Biol. 2007 Oct;27(20):7073-88. doi: 10.1128/MCB.02116-06. Epub 2007 Aug 6. Mol Cell Biol. 2007. PMID: 17682056 Free PMC article.
-
Dysfunctional mitochondrial fission impairs cell reprogramming.
Prieto J, León M, Ponsoda X, García-García F, Bort R, Serna E, Barneo-Muñoz M, Palau F, Dopazo J, López-García C, Torres J. Prieto J, et al. Cell Cycle. 2016 Dec;15(23):3240-3250. doi: 10.1080/15384101.2016.1241930. Epub 2016 Oct 18. Cell Cycle. 2016. PMID: 27753531 Free PMC article.
-
Redundant and distinct functions for dynamin-1 and dynamin-2 isoforms.
Altschuler Y, Barbas SM, Terlecky LJ, Tang K, Hardy S, Mostov KE, Schmid SL. Altschuler Y, et al. J Cell Biol. 1998 Dec 28;143(7):1871-81. doi: 10.1083/jcb.143.7.1871. J Cell Biol. 1998. PMID: 9864361 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases