The chromaffin granule surface. Localization of carbohydrate on the cytoplasmic surface of an intracellular organelle - PubMed

The chromaffin granule surface. Localization of carbohydrate on the cytoplasmic surface of an intracellular organelle

D I Meyer et al. Biochim Biophys Acta. 1976.

Abstract

Intact chromaffin granules from bovine adrenal medulla are shown to have complex carbohydrates on their external (cytoplasmic) surface. This is demonstrated by the facts (1) that granules can be agglutinated by wheat germ agglutinin, and (2) that significant amounts of sialic acid can be removed from the granule surface with neuraminidase. Glycoproteins located in the granule membrane, and not glycolipids, are the molecules that mediate wheat germ agglutinin agglutination. The possible involvement of granule surface carbohydrate in the process of exocytosis is discussed.

Similar articles

• Membranes of chromaffin granules. Isolation and partial characterization of two proteins.

  Hörtnagl H, Winkler H, Schöpf JA, Hohenwallner W. Hörtnagl H, et al. Biochem J. 1971 Apr;122(3):299-304. Biochem J. 1971. PMID: 5118102 Free PMC article.

• The ionogenic nature of the secretory-granule membrane. Electrokinetic properties of isolated chromaffin granules.

  Matthews EK, Evans RJ, Dean PM. Matthews EK, et al. Biochem J. 1972 Dec;130(3):825-32. doi: 10.1042/bj1300825. Biochem J. 1972. PMID: 4664936 Free PMC article.

• Membranes of the adrenal medulla. Behaviour of insoluble proteins of chromaffin granules on gel electrophoresis.

  Winkler H, Hörtnagl H, Smith AD. Winkler H, et al. Biochem J. 1970 Jun;118(2):303-10. doi: 10.1042/bj1180303. Biochem J. 1970. PMID: 4320820 Free PMC article.

• [Biology of lectins and their application in clinical biochemistry (author's transl)].

  Köttgen E. Köttgen E. Klin Wochenschr. 1977 Apr 15;55(8):359-73. doi: 10.1007/BF01488621. Klin Wochenschr. 1977. PMID: 323566 Review. German.

• The lamellar granules of epidermis.

  Odland GF, Holbrook K. Odland GF, et al. Curr Probl Dermatol. 1981;9:29-49. doi: 10.1159/000403343. Curr Probl Dermatol. 1981. PMID: 7023851 Review. No abstract available.

Cited by

• Comparison of chemical properties of purified plasma membranes and secretory vesicle membranes from bovine adrenal medulla.

  Zinder O, Hoffman PG, Bonner WM, Pollard HB. Zinder O, et al. Cell Tissue Res. 1978 Apr 17;188(2):153-70. doi: 10.1007/BF00222628. Cell Tissue Res. 1978. PMID: 647746 No abstract available.

• Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.

  Holt GD, Snow CM, Senior A, Haltiwanger RS, Gerace L, Hart GW. Holt GD, et al. J Cell Biol. 1987 May;104(5):1157-64. doi: 10.1083/jcb.104.5.1157. J Cell Biol. 1987. PMID: 3571327 Free PMC article.

• A monoclonal antibody against a family of nuclear pore proteins (nucleoporins): O-linked N-acetylglucosamine is part of the immunodeterminant.

  Park MK, D'Onofrio M, Willingham MC, Hanover JA. Park MK, et al. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6462-6. doi: 10.1073/pnas.84.18.6462. Proc Natl Acad Sci U S A. 1987. PMID: 2442757 Free PMC article.

MeSH terms

Substances