Soluble ICAM-1 activates lung macrophages and enhances lung injury - PubMed

Affiliations

• PMID: 9759893

Soluble ICAM-1 activates lung macrophages and enhances lung injury

H Schmal et al. J Immunol. 1998.

Abstract

Because of the important role of rat ICAM-1 in the development of lung inflammatory injury, soluble recombinant rat ICAM-1 (sICAM-1) was expressed in bacteria, and its biologic activities were evaluated. Purified sICAM-1 did bind to rat alveolar macrophages in a dose-dependent manner and induced production of TNF-alpha and the CXC chemokine, macrophage inflammatory protein-2 (MIP-2). Alveolar macrophages exhibited cytokine responses to both sICAM-1 and immobilized sICAM-1, while rat PBMCs failed to demonstrate similar responses. Exposure of alveolar macrophages to sICAM-1 resulted in NFkappaB activation (which was blocked by the presence of the aldehyde peptide inhibitor of 28S proteosome and by genistein, a tyrosine kinase inhibitor). As expected, cross-linking of CD18 on macrophages with Ab resulted in generation of TNF-alpha and MIP-2. This response was also inhibited in the presence of the proteosome inhibitor and by genistein. Alveolar macrophages showed adherence to immobilized sICAM-1 in a CD18-dependent manner. Finally, airway instillation of sICAM-1 intensified lung injury produced by intrapulmonary deposition of IgG immune complexes in a manner associated with enhanced lung production of TNF-alpha and MIP-2 and increased neutrophil recruitment. Therefore, through engagement of beta2 integrins, sICAM-1 enhances alveolar macrophage production of MIP-2 and TNF-alpha, the result of which is intensified lung injury after intrapulmonary disposition of immune complexes.

Similar articles

• In vitro and in vivo dependency of chemokine generation on C5a and TNF-alpha.

  Czermak BJ, Sarma V, Bless NM, Schmal H, Friedl HP, Ward PA. Czermak BJ, et al. J Immunol. 1999 Feb 15;162(4):2321-5. J Immunol. 1999. PMID: 9973510

• Requirements for alpha d in IgG immune complex-induced rat lung injury.

  Shanley TP, Warner RL, Crouch LD, Dietsch GN, Clark DL, O'Brien MM, Gallatin WM, Ward PA. Shanley TP, et al. J Immunol. 1998 Jan 15;160(2):1014-20. J Immunol. 1998. PMID: 9551942

• Neutrophil emigration in the lungs.

  Doerschuk CM, Kumasaka T, Qin L, Kutkoski GJ, Kubo H, Doyle NA, Quinlan WM. Doerschuk CM, et al. Nihon Kyobu Shikkan Gakkai Zasshi. 1996 Dec;34 Suppl:141-5. Nihon Kyobu Shikkan Gakkai Zasshi. 1996. PMID: 9216204 Review.

• On the relationship between activation and breakdown of macrophages in the pathogenesis of silicosis (an overview).

  Privalova LI, Katsnelson BA, Sharapova NY, Kislitsina NS. Privalova LI, et al. Med Lav. 1995 Nov-Dec;86(6):511-21. Med Lav. 1995. PMID: 8815362 Review.

Cited by

• Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia.

  Paetau S, Rolova T, Ning L, Gahmberg CG. Paetau S, et al. Front Mol Neurosci. 2017 Dec 22;10:431. doi: 10.3389/fnmol.2017.00431. eCollection 2017. Front Mol Neurosci. 2017. PMID: 29311819 Free PMC article.

• Elevated plasma ICAM1 levels predict 28-day mortality in cirrhotic patients with COVID-19 or bacterial sepsis.

  Kaur S, Hussain S, Kolhe K, Kumar G, Tripathi DM, Tomar A, Kale P, Narayanan A, Bihari C, Bajpai M, Maiwall R, Gupta E, Sarin SK. Kaur S, et al. JHEP Rep. 2021 Aug;3(4):100303. doi: 10.1016/j.jhepr.2021.100303. Epub 2021 May 8. JHEP Rep. 2021. PMID: 33997748 Free PMC article.

• Association of T Cell and Macrophage Activation with Arterial Vascular Health in HIV.

  Grome HN, Barnett L, Hagar CC, Harrison DG, Kalams SA, Koethe JR. Grome HN, et al. AIDS Res Hum Retroviruses. 2017 Feb;33(2):181-186. doi: 10.1089/AID.2016.0113. Epub 2016 Sep 14. AIDS Res Hum Retroviruses. 2017. PMID: 27527002 Free PMC article.

• The influence of tobacco smoking on adhesion molecule profiles.

  Scott DA, Palmer RM. Scott DA, et al. Tob Induc Dis. 2002 Jan 15;1(1):7-25. doi: 10.1186/1617-9625-1-1-7. Tob Induc Dis. 2002. PMID: 19570245 Free PMC article.

• Bench-to-bedside: The use of local anesthetics to attenuate inflammation in acute respiratory distress syndrome.

  Krishnamoorthy V, Chung L. Krishnamoorthy V, et al. Int J Crit Illn Inj Sci. 2014 Apr;4(2):98-100. doi: 10.4103/2229-5151.134143. Int J Crit Illn Inj Sci. 2014. PMID: 25024936 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal
  • SciCrunch