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Chloroplast division in higher plants requires members of two functionally divergent gene families with homology to bacterial ftsZ - PubMed

Comparative Study

Chloroplast division in higher plants requires members of two functionally divergent gene families with homology to bacterial ftsZ

K W Osteryoung et al. Plant Cell. 1998 Dec.

Abstract

The division of plastids is critical for viability in photosynthetic eukaryotes, but the mechanisms associated with this process are still poorly understood. We previously identified a nuclear gene from Arabidopsis encoding a chloroplast-localized homolog of the bacterial cell division protein FtsZ, an essential cytoskeletal component of the prokaryotic cell division apparatus. Here, we report the identification of a second nuclear-encoded FtsZ-type protein from Arabidopsis that does not contain a chloroplast targeting sequence or other obvious sorting signals and is not imported into isolated chloroplasts, which strongly suggests that it is localized in the cytosol. We further demonstrate using antisense technology that inhibiting expression of either Arabidopsis FtsZ gene (AtFtsZ1-1 or AtFtsZ2-1) in transgenic plants reduces the number of chloroplasts in mature leaf cells from 100 to one, indicating that both genes are essential for division of higher plant chloroplasts but that each plays a distinct role in the process. Analysis of currently available plant FtsZ sequences further suggests that two functionally divergent FtsZ gene families encoding differentially localized products participate in chloroplast division. Our results provide evidence that both chloroplastic and cytosolic forms of FtsZ are involved in chloroplast division in higher plants and imply that important differences exist between chloroplasts and prokaryotes with regard to the roles played by FtsZ proteins in the division process.

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References

    1. J Bacteriol. 1997 Jul;179(13):4277-84 - PubMed
    1. Plant Mol Biol. 1992 May;19(1):15-38 - PubMed
    1. Proc Natl Acad Sci U S A. 1997 May 27;94(11):5967-72 - PubMed
    1. Anal Biochem. 1987 May 15;163(1):16-20 - PubMed
    1. Annu Rev Biochem. 1997;66:93-116 - PubMed

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