Simultaneous recording of fluorescence and electrical signals by photometric patch electrode in deep brain regions in vivo
“…In order to overcome the limitations of diffraction or the depth of tissue penetration, multiphoton microscopy or super-resolution fluorescence microscopy has been widely applied for tissue observation . A multiphoton microscope has high tissue-penetration abilities. A super-resolution fluorescence microscope can overcome the diffraction limit, improving spatial resolution at the level of tens of nanometers. Both microscopes have greatly improved spatial resolution and depth. However, they require a fluorescent label, and the issue of noninvasive observation persists.…”
Section: Introductionmentioning
See 1 more Smart Citation
“…In order to overcome the limitations of diffraction or the depth of tissue penetration, multiphoton microscopy or super-resolution fluorescence microscopy has been widely applied for tissue observation . A multiphoton microscope has high tissue-penetration abilities. A super-resolution fluorescence microscope can overcome the diffraction limit, improving spatial resolution at the level of tens of nanometers. Both microscopes have greatly improved spatial resolution and depth. However, they require a fluorescent label, and the issue of noninvasive observation persists.…”
Section: Introductionmentioning
“…Hirai’s group was able to approach specific cells and record fluorescence and electrophysiological signals at a depth of 3–7 mm from the brain surface with a Ni-modified probe. They stimulated nuclei located at a depth of 3–7 mm from the brain surface, and successfully measured the calcium fluorescence and electrical signals synchronizing the stimulations . According to these results, detection of specific cells in a tissue and measuring electrophysiological signals were achieved; however, a technique that can visualize the 3D topography of the cell surface in a tissue has not been reported.…”
Section: Introductionmentioning