A Novel Expansin Protein from the White-Rot Fungus Schizophyllum commune

“…The preliminary results obtained in this study, derived via light microscopy analysis, showed a tendency toward deagglomeration of the Avicel structure in treatments that included purified recombinant ThSwo (Additional file 2: Figure S1). However, although our light microscopy findings were not conclusive, especially due to the crystalline proprieties of Avicel, the effects observed herein were similar to those reported for a previously characterized expansin-related protein [35, 42]. Meanwhile, the disruptive activity of ThSwo was confirmed by scanning electron microscopy (Fig.…”

“…and expansin‐like genes (10 of 21 genes) were likewise developmentally regulated in both species. Cerato‐platanins and expansins were mostly associated with the plant cell wall before (Baccelli, ; Tovar‐Herrera et al ., ), but their dynamic expression in fruiting bodies observed here and in previous studies (Sipos et al ., ; Krizsán et al ., ) suggests potential FCW‐related roles. Several putatively FCW‐active CAZymes (Fig.…”

“…We found 7 proteins containing CMB50 domains; 5 of 7 such proteins were increased after harvest, and 2 of 5 were identified as DEGs in the fruiting body after harvest (g6653.t1 and g8231.t1) ( Table 4). Expansin is involved in loosening cellulose crystal structures for cellulose degradation (51), and an expansinlike protein in Schizophyllum commune enhances chitin degradation (52). The expression of several expansin-like proteins, such as those encoded by baw28 (g7005.t1) and g6097.t1, increased after harvest.…”