EBV-miR-BART10-3p facilitates epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma by targeting BTRC

“…Further experiments showed that the increase in cell motility and invasiveness by EBV miR-BART9 was indeed associated with several mesenchymal EMT properties, namely, E-cadherin downregulation, induction of β-catenin signaling, expression of vimentin and MMPs, and spindle-cell morphology [80]. Essentially the same properties reported for EBV miR-BART9 were found for viral miR-BART10-3p, which expression was directly associated with patient poor survival in a series of 106 NPCs [81]. …”

“…For gene knockdown, cells were seed in six-well plates to confluency and transfected siRNAs by using Lipofectamine RNAiMAX Reagent (Invitrogen) in OptiMEM medium (Invitrogen) [58–61]. Two sequences of LINC00673 targeting siRNAs were: 5′-CCAGTTGTC CTTGACTGCATGGTTT-3′; and 5′-AGGGAACCACAGGATTCCATGTGAT-3′.…”

“…Many assays have respectively demonstrated ECM components, including collagens 245-247, laminins 248, fibronectins 249, proteoglycans 250, and hyaluronans 251, 252 in a specific organization 225. In vitro, one three-dimensional (3D) model indicated that ECM heterogeneity is crucial for controlling collective cell invasive behaviors and determining metastasis efficiency 253-262. Because of the complexity of ECM heterogeneity in vivo, its influence on collective cell behavior has been described but not quantified 253.…”