Shared evolutionary origin of vertebrate neural crest and cranial placodes
“…The arrows show the delamination and dorsal migration of ESN precursors (Kaltenbach et al, 2009). In all lateral views, anterior is toward the left and dorsal toward the top BTNs can be transformed into palp sensory cells, a proto-placodal sensory cell type that arises from the anterior-most regions of the Ciona NPB (Horie et al, 2018). These results demonstrate the common origin of NPB in Olfactores (vertebrates and tunicates).…”
Section: Pn S Neuron S Derived From Primitive Neur Al Cre S T and Pmentioning
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“…The arrows show the delamination and dorsal migration of ESN precursors (Kaltenbach et al, 2009). In all lateral views, anterior is toward the left and dorsal toward the top BTNs can be transformed into palp sensory cells, a proto-placodal sensory cell type that arises from the anterior-most regions of the Ciona NPB (Horie et al, 2018). These results demonstrate the common origin of NPB in Olfactores (vertebrates and tunicates).…”
Section: Pn S Neuron S Derived From Primitive Neur Al Cre S T and Pmentioning
“…For example, Ciona and vertebrate NPB share sequential expression patterns of Six1/2 , Pax3/7 and Msxb , corresponding to progenitor domains of placode and neural crest, respectively. Dramatically, BTNs can be transformed into palp sensory cells, a proto‐placodal sensory cell type that arises from the anterior‐most regions of the Ciona NPB (Horie et al., ). These results demonstrate the common origin of NPB in Olfactores (vertebrates and tunicates).…”
Section: Pns Neurons Derived From Primitive Neural Crest and Placodementioning
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“…Since centrosome repositioning has been shown to depend on microtubule stabilization (Pitaval et al, 2017) the repositioning of ddN centrosomes that we observe might be effected in part by microtubule stabilization, driven by ddN-specific upregulation of Saxo and/or Nckap5 (see follow up experiments below). Saxo transcripts were also detected in migrating BTNs by ISH (Fig 3A), and by single-cell RNAseq analysis (Horie et al, 2018a), hinting at the possible involvement of Saxo in centrosome repositioning in BTNs too.…”
Section: Centrosome-enriched Proteins Are Upregulated In Ddnsmentioning
“…To see if we were measuring meaningful expression of Neurog targets in the BTNs. We crossreferenced this differential gene expression data to a previously published single-cell RNAseq data set comprising the top 100 genes enriched in the BTNs relative to other cell types at 12 hpf at 18°C (Horie et al, 2018) (Supplemental Table 2), with the exception of two genes: KH.S1555.2 (which was not present in our dataset) and Neurog (due to confounding reads from the electroporated plasmids). We found that all 98 top BTN genes in the scRNAseq dataset were positively regulated by Neurog overexpression (LogFC>0, Fig 3c).…”
mentioning