Nitrosomonas supralitoralis sp. nov., an ammonia-oxidizing bacterium from beach sand in a supralittoral zone

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• Andrews, Gabrianna A.
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• Lopez, Jose V.
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• Martens-Habbena, Willm
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• Klotz, Martin G.
;
• Stahl, David A.

Abstract

A betaproteobacterial chemolithotrophic ammonia-oxidizing bacterium designated APG5^T was isolated from supralittoral sand of the Edmonds City Beach, WA, USA. Growth was observed at 10–35 °C (optimum, 30 °C), pH 5–9 (optimum, pH 8) and ammonia concentrations as high as 100 mM (optimum, 1–30 mM NH₄Cl). The strain grows optimally in a freshwater medium but tolerates up to 400 mM NaCl. It is most closely related to 'Nitrosomonas ureae' (96.7% 16S rRNA and 92.4% amoA sequence identity). The 3.75-Mbp of AGP5^T draft genome contained a single rRNA operon and all necessary tRNA genes and has the lowest G+C content (43.5%) when compared to the previously reported genomes of reference strains in cluster 6 Nitrosomonas. Based on an average nucleotide identity of 82% with its closest relative ('N. ureae' Nm10^T) and the suggested species boundary of 95–96%, a new species Nitrosomonas supralitoralis sp. nov. is proposed. The type strain of Nitrosomonas supralitoralis is APG5^T (= NCIMB 14870^T = ATCC TSD-116^T).