GMO Detection Method Database (GMDD)
- ️tfmao
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| Introduced Elements | ||||||||||||||||||||||||||||||||||
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| Target Gene | ||||||||||||||||||||||||||||||||||
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As mentioned in the Monsanto's original petition, GTS40-3-2 contains a single insert, containing P-35S, CTP4, cp4 epsps, and T-nos. No other elements are found in the cotton genome.
Additional studies conducted by Monsanto showed that the insert of T-nos is complete. A 250 bp segment of the cp4 epsps, and a 534 bp rearrangement of the DNA are found adjacent to the 3' end of the T-nos. Besides, a 72 bp cp4 epsps inactive fragment is also found in the genome.
Nucleic Acid - Based Method For Exogenous Gene:| Strategy | Method Type | Primer Name | Amplicon | Probe Type | Validation Info | Standard |
| Screening | Qualitative | P35S-cf3 / P35S-cr4 | 123bp | Yes | ISO | |
| HA-nos118-f / HA-nos118-r | 118bp | Yes | ISO | |||
| tNOS 2-5 / tNOS 2-3 | 151bp | |||||
| 35s-1 / 35s-2 | 195bp | Yes | ISO | |||
| 35SFZMP1 / 35SFZMP2 | 158bp | |||||
| nosFZMP1 / nosFZMP2 | 125bp | |||||
| Quantitative | 35S-F / 35S-R | 83bp | TaqMan | Yes | ISO&National; |