| Entry |
EC 1.8.5.5 Enzyme
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| Name |
thiosulfate reductase (quinone);
phsABC (gene names)
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| Class |
Oxidoreductases;
Acting on a sulfur group of donors;
With a quinone or similar compound as acceptor
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| Sysname |
sulfite,hydrogen sulfide:quinone oxidoreductase
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| Reaction(IUBMB) |
sulfite + hydrogen sulfide + a quinone = thiosulfate + a quinol [RN:R11488]
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| Reaction(KEGG) |
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| Substrate |
sulfite [CPD:C00094];
hydrogen sulfide [CPD:C00283];
quinone [CPD:C15602]
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| Product |
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| Comment |
The enzyme, characterized from the bacterium Salmonella enterica, is similar to EC 1.17.5.3, formate dehydrogenase-N. It contains a molybdopterin-guanine dinucleotide, five [4Fe-4S] clusters and two heme b groups. The reaction occurs in vivo in the direction of thiosulfate disproportionation, which is highly endergonic. It is driven by the proton motive force that occurs across the cytoplasmic membrane.
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| History |
EC 1.8.5.5 created 2016, modified 2017
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K08352 | thiosulfate reductase / polysulfide reductase chain A |
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| Genes |
| LRI: | NCTC12151_02197(psrA) |
| SCAA: | TUM17387_33610(phsA) |
| SYOU: | RGE70_14000(phsA) RGE70_16200(phsA) |
| TNI: | TVNIR_0949(phsA_[H]) |
| SEDS: | AAY24_06365 AAY24_12230 |
| SUTT: | SUTMEG_11480 SUTMEG_15000 |
| RBH: | B4966_04570 B4966_04605 |
| HPUL: | NCTC13154_00091(psrA) |
| WSU: | WS0116(fdhA) WS0709(fdhA) |
| SDL: | Sdel_0265 Sdel_0269 Sdel_2276 |
| SBA: | Sulba_0281 Sulba_0285 Sulba_2546 |
| SMUL: | SMUL_0342(psrA) SMUL_0346 SMUL_3275 SMUL_3282 |
| SHAL: | SHALO_0307 SHALO_0311 SHALO_2994 SHALO_3001 |
| SULS: | Sdiek1_0301 Sdiek1_0305 Sdiek1_2974 Sdiek1_2981 |
| SULJ: | SJPD1_0305 SJPD1_0309 SJPD1_2864 SJPD1_2871 |
| SULT: | FA592_07095 FA592_08605(phsA) FA592_08625(phsA) |
| AHS: | AHALO_0201 AHALO_2400 |
| AMAR: | AMRN_0240 AMRN_2501 |
| ACAA: | ACAN_0252 ACAN_2514 |
| AMOL: | AMOL_1120 AMOL_1900 |
| HEBR: | AEBR_0305 AEBR_1184 |
| HTM: | RZR91_12110 RZR91_12125 |
| SINU: | IMZ28_06785 IMZ28_07170 IMZ28_07270 |
| PSYF: | N1030_03730 N1030_03750 |
| DSB: | LN040_01670 LN040_01690 |
| DAF: | Desaf_1489 Desaf_1628 Desaf_3550 |
| DEJ: | AWY79_01170 AWY79_07465 |
| PPRF: | DPRO_1730 DPRO_3417 |
| PSEL: | GM415_04510 GM415_09450 |
| DDN: | DND132_0424 DND132_1314 DND132_2597 |
| PSEF: | PSDVSF_12760 PSDVSF_25560 |
| PPOR: | JCM14722_09970 JCM14722_11410 JCM14722_20920 |
| PNW: | SYK_14370 SYK_14810 SYK_22390 SYK_26190 |
| PTHE: | LF599_01615 LF599_07235 LF599_13910 |
| PMEN: | V8V93_04345 V8V93_16970 |
| DSX: | GD604_01660 GD604_16610 |
| DSD: | GD606_10630 GD606_17415 |
| CLIH: | KPS_000190 KPS_000421 |
| DBA: | Dbac_0171 Dbac_2338 Dbac_2860 |
| DESU: | NLA06_10375 NLA06_12095 NLA06_14260 |
| DSF: | UWK_01205 UWK_02642 UWK_02791 |
| DAK: | DaAHT2_0420 DaAHT2_0547 |
| DPR: | Despr_1135 Despr_1859 Despr_2399 |
| DOG: | HP555_09720 HP555_14005 |
| DBK: | DGMP_14050 DGMP_21170 DGMP_32000 |
| DDU: | GF1_06580 GF1_10120 GF1_30490 |
| ECOM: | QTN59_06890 QTN59_13425 |
| EAJ: | Q3M24_05095 Q3M24_16985 |
| ELAX: | KKHLCK_05770 KKHLCK_13045 |
| EAQ: | LDFHOB_07320 LDFHOB_12305 |
| DOV: | DSCO28_04200 DSCO28_16650 DSCO28_54490 |
| DWD: | DSCW_28610 DSCW_31190 |
| DALK: | DSCA_26800 DSCA_49610 DSCA_57040 DSCA_61680 |
| DTI: | Desti_1387 Desti_1738 |
| TCM: | HL41_04470 HL41_08650 |
| THET: | F1847_03060 F1847_07855 |
| TAV: | G4V39_07125 G4V39_08920 |
| AORY: | AMOR_50280 AMOR_50350 |
| PROC: | Ptc2401_02170(psrA) |
| DDF: | DEFDS_0670 DEFDS_1699 |
| DAP: | Dacet_1678 Dacet_1793 |
| GTL: | EP073_09415 EP073_11320 |
| FSI: | Flexsi_1504 Flexsi_1512 |
| TYE: | THEYE_A1128 THEYE_A1157 |
| TOB: | V4D31_04890 V4D31_05390 |
| TAUT: | V4D30_02210 V4D30_02810 |
| DHR: | LGS26_06635 LGS26_09210 |
| MJO: | FOF60_05470(srrA) FOF60_09180 |
| MSUT: | LC048_12440 LC048_16385(srrA) |
| CMIC: | caldi_16080 caldi_16110 |
| DDL: | Desdi_1252 Desdi_2963 |
| AACX: | DEACI_1081 DEACI_3149 |
| ELE: | Elen_0442 Elen_0447 Elen_2753 Elen_2919 |
| EGD: | GS424_002570 GS424_002595 GS424_016830 |
| AHAT: | ADCFC_03680 ADCFC_20030 |
| CBAC: | JI75_05060 JI75_08700 |
| BWA: | HLV38_01315 HLV38_05530 |
| RTS: | CE91St31_28640 CE91St31_29860 |
| HAHS: | HSRCO_0103(bisC) HSRCO_1045(bisC5) |
| HARA: | AArcS_0641(bisC) AArcS_0990(bisC2) |
| HDS: | HSR122_1817(bisC) HSR122_2527(bisC2) HSR122_2992(bisC4) |
| HDF: | AArcSl_0127(phsA) AArcSl_1905(phsA2) |
| NGE: | Natgr_0551 Natgr_2276 |
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| Reference |
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| Authors |
Kwan HS, Barrett EL |
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| Title |
Map locations and functions of Salmonella typhimurium men genes. |
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| Journal |
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| Reference |
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| Authors |
Clark MA, Barrett EL |
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| Title |
The phs gene and hydrogen sulfide production by Salmonella typhimurium. |
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| Journal |
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| Reference |
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| Authors |
Alami N, Hallenbeck PC |
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| Title |
Cloning and characterization of a gene cluster, phsBCDEF, necessary for the production of hydrogen sulfide from thiosulfate by Salmonella typhimurium. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Heinzinger NK, Fujimoto SY, Clark MA, Moreno MS, Barrett EL |
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| Title |
Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Stoffels L, Krehenbrink M, Berks BC, Unden G |
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| Title |
Thiosulfate reduction in Salmonella enterica is driven by the proton motive force. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 1.8.5.5 |
| ExPASy - ENZYME nomenclature database: | 1.8.5.5 |
| BRENDA, the Enzyme Database: | 1.8.5.5 |
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| LinkDB |
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