Figure 2 | Cell Research
Interactions of Stat1 with hBrm and Brg1. (A) Schematic drawing of the wild-type and truncated Stat1 fragments. “+” or “−”: results of interactions between Stat1 (fragments) and hBrm or Brg1. Bottom panel: Co-IP assays with Jurkat cells transfected with HA-tagged Stat1 (S1-S6) or its vector, as control (vec). (B) co-IP of NE from Jurkat cells. IP: antibody used for immunoprecipitation; IB: antibodies used for immunoblotting; S: negative control; “+” or “−” indicates with or without IP. (C) Co-IP of proteins recovered from chromatin fragments and NE of Jurkat cells. Input: total chromatin fragments. (D) Western blot analysis of the cytoplasmic and nuclear fractions of Jurkat cells. pY-Stat1: phosphorylated Stat1 at Y701; pS-Stat1: phosphorylated Stat1 at S727; H5, H10, H20, H30 and H60: 42 °C for 5, 10, 20, 30 and 60 min, respectively. (E, F) Co-IP assays on FLAG-tagged mutants of Stat1 pre-transfected Jurkat cells. WT: wild-type Stat1; Y/F and S/A: Y701F- and S727A-Stat1; IP: antibody against FLAG; C: 37 °C; H: 42 °C for 60 min.