Figure 6 | Cell Research
The hBrm-to-Brg1 switch at the respective GAS elements of hsp90α, CIITA and BCL6 and the activation of specific genes in Jurkat and Raji cells upon heat shock. (A, B) Effects of siRNA specific for Brg1 or hBrm on the endogenous mRNA expression of CIITA and BCL6 in Jurkat (A) and of hsp90α in Raji (B) cells. Total RNA was extracted and quantified by qRT-PCR. (C, D) ChIP analysis showing the recruitment of endogenous Stat1, hBrm and Brg1 at the respective GAS regions of the hsp90α, pIV-CIITA and BCL6 genes in Jurkat (C) and Raji (D) cells.