Figure 1: TWS119 activates Wnt signaling in CD8+ T cells. | Nature Medicine
Naive CD8+ T cells were primed in vitro with antibody to CD3 (2 μg ml−1) and antibody to CD28 (1 μg ml−1) with or without 7 μM TWS119. (a) Western blot analysis of β-catenin and glyceraldehyde-3-phosphate dehydrogenase (Gapdh) in CD8+ T cells treated with or without TWS119. (b) Electrophoretic mobility shift assay of nuclear extract from CD8+ T cells treated with or without TWS119 using P32-labeled oligonucleotide probes (oligo) designed from the Tcf and Lef binding region of the Tcf-1 target gene Fzd7. Unlabeled oligonucleotide probes were used as competitors. (c) Quantitative RT-PCR analysis of the expression of Tcf7, Lef1, Jun, Fzd7 and Nlk in CD8+ T cells treated with or without TWS119. Data are represented as means ± s.e.m. All data are representative of at least two independently performed experiments.