Figure 2: Wnt signaling inhibits CD8+ T cell proliferation and effector differentiation. | Nature Medicine
(a–c) Flow cytometry analysis. Numbers represent the percentage of cells in the dot-plot quadrants. (a) CFSE dilution assays (b) and cytokine and 51Cr release assays (c) of CFSE-labeled naive pmel-1 CD8+ T cells primed in vitro with CD8+ T cell depleted splenocytes pulsed with 1 μM hgp10025–33, in conjunction with 10 ng ml−1 IL-2 and titrated doses of TWS119, 4 d after T cell activation. Data in c are represented as means ± s.e.m. E:T, effector to target ratio. (d) Quantitative RT-PCR analysis of the expression of Eomes in CD8+ T cells after priming with CD3-specific and CD28-specific antibodies with or without 7 μM TWS119. Data are represented as means ± s.e.m. (e) Flow cytometry and enumeration of T cell subsets 6 d after vaccination with or without TWS119. WT mice received adoptive transfer of 1 × 106 naive pmel-1 Thy1.1+ CD8+ T cells in conjunction with recombinant fowlpox-based hgp100 vaccine. Mice received four daily doses of TWS119 (at 30 mg kg−1) from day 0 to day 3 or DMSO as control. All data are representative of at least two independently performed experiments.