Extended Data Fig. 2: Analysis of IgA- and IgE-bound bacteria in fecal samples. | Nature Medicine
a,c, Representative FACS plots showing the gating strategy for human (a) and mouse (c) fecal bacteria. Bacteria present in the feces were identified by gating on SYTO-BC+ events (right-side panels). b,d, Frequencies of IgA- and IgE-bound bacteria as assessed by gating on bacteria bound with the respective PE-labeled anti-IgA and anti-IgE antibodies, as shown in a and c. e,f, Analysis of sIgA+ (e) and IgE+ (f) fecal bacteria from Il4raF709 mice sensitized with OVA/SEB without or with bacterial therapy. Fecal pellets from Rag2–/– and Igh7–/–Il4raF709 mice were used as negative controls. Each symbol in the scatter plots represents one mouse (no treatment: n = 11 per group; Clostridiales: n = 8 per group; Proteobacteria: n = 9 and 7). Data represent mean ± s.e.m. from two independent experiments. Flow panels in c,d are representative of two two independent experiments. P values were derived by one-way ANOVA with Dunnett’s post hoc analysis.