Figure 6 | Scientific Reports

Aspirin inhibited de novo protein synthesis.

(a) Ingenuity Pathway Analysis™ (IPA) revealing that aspirin affects gene expression and protein synthesis. All proteins shown as green nodes were identified as the specific targets of aspirin. (b) Aspirin inhibited de novo protein synthesis in HCT116 cells and MEFs. Cells were firstly labelled with 50 μM AHA in L-methionine-free medium with or without aspirin (5 mM) treatment for 8 hrs or 16 hrs. The cells were then harvested, fixed and permeabilized for the click reaction. Finally, cellular fluorescence intensity was analysed using flow cytometry. Data for the relative signal intensity was expressed as the ratio of treated cells to control cells, as mean ± SD from three independent experiments; * P < 0.05, ** P < 0.01, derived from the Student's t-test.