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  • ️Wed Feb 05 2025

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  • Lineage tracing of stem cells decades after blood and bone-marrow transplantation

    In individuals receiving an allogeneic haematopoietic cell transplant, stem cells in the bone marrow, which give rise to blood and immune cells, are replaced with those from a donor. Genome-sequencing analyses in donor–recipient pairs years after transplantation show an accelerated loss of clonal diversity in the blood stem cells of recipients compared with those of donors.

    News & Views05 Feb 2025 Nature

  • Single-cell profiling of the immune landscape across the human lifespan

    Using a combination of single-cell RNA sequencing, T cell and B cell receptor sequencing, and mass cytometry analyses, we characterized the dynamics of peripheral immune cells across the human lifespan. Based on this resource, we present a single-cell immune aging clock to assess an individual’s immune status across different life stages.

Related Subjects

Latest Research and Reviews

  • Linking single-cell transcriptomes with secretion using SEC-seq

    SEC-seq is a single-cell method linking the secretion of a target protein to the cell’s transcriptome. Nanovials isolate cells and capture their secretions, and oligonucleotide-labeled antibodies tag them, followed by an scRNA-seq readout.

    • Justin Langerman
    • Sevana Baghdasarian
    • Dino Di Carlo

News and Comment

  • Lineage tracing of stem cells decades after blood and bone-marrow transplantation

    In individuals receiving an allogeneic haematopoietic cell transplant, stem cells in the bone marrow, which give rise to blood and immune cells, are replaced with those from a donor. Genome-sequencing analyses in donor–recipient pairs years after transplantation show an accelerated loss of clonal diversity in the blood stem cells of recipients compared with those of donors.

    News & Views05 Feb 2025 Nature

  • Unravelling the complexity of gene regulation through multiplexed protein mapping

    In this Tools of the Trade article, Goronzy (Guttman lab) describes the development of ChIP-DIP, a high-throughput, split-pool barcoding method that enables genome-wide profiling of hundreds of regulatory proteins, transforming our ability to study the regulation of gene activity across cell types and biological contexts.

    • Isabel Nadine Goronzy

  • Single-cell profiling of the immune landscape across the human lifespan

    Using a combination of single-cell RNA sequencing, T cell and B cell receptor sequencing, and mass cytometry analyses, we characterized the dynamics of peripheral immune cells across the human lifespan. Based on this resource, we present a single-cell immune aging clock to assess an individual’s immune status across different life stages.

  • The early days of transcriptome sequencing and functional genomics

    Ayako Suzuki recalls two papers published in the early 2000s by the Functional Annotation of the Mammalian Genome (FANTOM) consortium and the Full-Length Long Japan (FLJ) project, which provided reference catalogues of full-length transcripts in mice and humans, respectively.

    • Ayako Suzuki

  • The next generation of in situ multi-omics

    Spatial-Mux-seq adds to a growing portfolio of innovative spatial omics technologies and enables simultaneous profiling of up to five omic modalities in situ.

    • Maren Salla
    • Klara Penkert
    • Leif S. Ludwig