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Von Willebrand protein binds to extracellular matrices independently of collagen

Abstract

Von Willebrand protein is present in the extracellular matrix of endothelial cells where it codistributes with fibronectin and types IV and V collagen. Bacterial collagenase digestion of endothelial cells removed fibrillar collagen, but the pattern of fibronectin and of von Willebrand protein remained undisturbed. Exogenous von Willebrand protein bound to matrices of different cells, whether rich or poor in collagen. von Willebrand protein also decorated the matrix of cells grown in the presence of alpha, alpha'-dipyridyl, a collagen-secretion inhibitor. These results indicate that the presence of von Willebrand protein in the extracellular matrix of endothelial cells and the binding of von Willebrand protein to foreign matrices do not depend on the matrix collagen.

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Selected References

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  1. Alitalo K., Myllyla R., Sage H., Pritzl P., Vaheri A., Bornstein P. Biosynthesis of type V procollagen by A204, a human rhabdomyosarcoma cell line. J Biol Chem. 1982 Aug 10;257(15):9016–9024. [PubMed] [Google Scholar]
  2. Booyse F. M., Quarfoot A. J., Bell S., Fass D. N., Lewis J. C., Mann K. G., Bowie E. J. Cultured aortic endothelial cells from pigs with von Willebrand disease: in vitro model for studying the molecular defect(s) of the disease. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5702–5706. doi: 10.1073/pnas.74.12.5702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caen J. P., Nurden A. T., Jeanneau C., Michel H., Tobelem G., Levy-Toledano S., Sultan Y., Valensi F., Bernard J. Bernard-Soulier syndrome: a new platelet glycoprotein abnormality. Its relationship with platelet adhesion to subendothelium and with the factor VIII von Willebrand protein. J Lab Clin Med. 1976 Apr;87(4):586–596. [PubMed] [Google Scholar]
  4. Coller B. S., Peerschke E. I., Scudder L. E., Sullivan C. A. Studies with a murine monoclonal antibody that abolishes ristocetin-induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor. Blood. 1983 Jan;61(1):99–110. [PubMed] [Google Scholar]
  5. Fauvel F., Grant M. E., Legrand Y. J., Souchon H., Tobelem G., Jackson D. S., Caen J. P. Interaction of blood platelets with a microfibrillar extract from adult bovine aorta: requirement for von Willebrand factor. Proc Natl Acad Sci U S A. 1983 Jan;80(2):551–554. doi: 10.1073/pnas.80.2.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fay P. J., Chavin S. I., Schroeder D., Young F. E., Marder V. J. Purification and characterization of a highly purified human factor VIII consisting of a single type of polypeptide chain. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7200–7204. doi: 10.1073/pnas.79.23.7200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harper E., Berger A., Katchalski E. The hydrolysis of poly (L-Prolyl-Glycyl-L-Prolyl) by bacterial collagenase. Biopolymers. 1972;11(8):1607–1612. doi: 10.1002/bip.1972.360110807. [DOI] [PubMed] [Google Scholar]
  8. Hayman E. G., Oldberg A., Martin G. R., Ruoslahti E. Codistribution of heparan sulfate proteoglycan, laminin, and fibronectin in the extracellular matrix of normal rat kidney cells and their coordinate absence in transformed cells. J Cell Biol. 1982 Jul;94(1):28–35. doi: 10.1083/jcb.94.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoyer L. W. The factor VIII complex: structure and function. Blood. 1981 Jul;58(1):1–13. [PubMed] [Google Scholar]
  10. Hynes R. O., Destree A. T. Relationships between fibronectin (LETS protein) and actin. Cell. 1978 Nov;15(3):875–886. doi: 10.1016/0092-8674(78)90272-6. [DOI] [PubMed] [Google Scholar]
  11. Hynes R. O., Humphryes K. C. Characterization of the external proteins of hamster fibroblasts. J Cell Biol. 1974 Aug;62(2):438–448. doi: 10.1083/jcb.62.2.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jaffe E. A., Hoyer L. W., Nachman R. L. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest. 1973 Nov;52(11):2757–2764. doi: 10.1172/JCI107471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jenkins C. S., Phillips D. R., Clemetson K. J., Meyer D., Larrieu M. J., Lüscher E. F. Platelet membrane glycoproteins implicated in ristocetin-induced aggregation. Studies of the proteins on platelets from patients with Bernard-Soulier syndrome and von Willebrand's disease. J Clin Invest. 1976 Jan;57(1):112–124. doi: 10.1172/JCI108251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jimenez S. A., Dehm P., Olsen B. R., Prokop D. J. Intracellular collagen and protocollagen from embryonic tendon cells. J Biol Chem. 1973 Jan 25;248(2):720–729. [PubMed] [Google Scholar]
  15. Kao K. J., Pizzo S. V., McKee P. A. Demonstration and characterization of specific binding sites for factor VIII/von Willebrand factor on human platelets. J Clin Invest. 1979 Apr;63(4):656–664. doi: 10.1172/JCI109348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koutts J., Walsh P. N., Plow E. F., Fenton J. W., 2nd, Bouma B. N., Zimmerman T. S. Active release of human platelet factor VIII-related antigen by adenosine diphosphate, collagen, and thrombin. J Clin Invest. 1978 Dec;62(6):1255–1263. doi: 10.1172/JCI109246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Laurie G. W., Leblond C. P., Martin G. R. Localization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin to the basal lamina of basement membranes. J Cell Biol. 1982 Oct;95(1):340–344. doi: 10.1083/jcb.95.1.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Martin S. E., Marder V. J., Francis C. W., Loftus L. S., Barlow G. H. Enzymatic degradation of the factor-VIII-von-Willebrand protein: a unique tryptic fragment with ristocetin cofactor activity. Blood. 1980 May;55(5):848–858. [PubMed] [Google Scholar]
  19. Nachman R., Levine R., Jaffe E. A. Synthesis of factor VIII antigen by cultured guinea pig megakaryocytes. J Clin Invest. 1977 Oct;60(4):914–921. doi: 10.1172/JCI108846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Perkins M. E., Ji T. H., Hynes R. O. Cross-linking of fibronectin to sulfated proteoglycans at the cell surface. Cell. 1979 Apr;16(4):941–952. doi: 10.1016/0092-8674(79)90109-0. [DOI] [PubMed] [Google Scholar]
  21. Ramaley P. B., Rosenbloom J. Inhibition of proline and lysine hydroxylation prevents normal extrusion of collagen by 3T6 fibroblasts in culture. FEBS Lett. 1971 Jun 2;15(1):59–64. doi: 10.1016/0014-5793(71)80079-0. [DOI] [PubMed] [Google Scholar]
  22. Rand J. H., Gordon R. E., Sussman I. I., Chu S. V., Solomon V. Electron microscopic localization of factor-VIII-related antigen in adult human blood vessels. Blood. 1982 Sep;60(3):627–634. [PubMed] [Google Scholar]
  23. Rand J. H., Sussman I. I., Gordon R. E., Chu S. V., Solomon V. Localization of factor-VIII-related antigen in human vascular subendothelium. Blood. 1980 May;55(5):752–756. [PubMed] [Google Scholar]
  24. Raugi G. J., Mumby S. M., Abbott-Brown D., Bornstein P. Thrombospondin: synthesis and secretion by cells in culture. J Cell Biol. 1982 Oct;95(1):351–354. doi: 10.1083/jcb.95.1.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ruan C., Tobelem G., McMichael A. J., Drouet L., Legrand Y., Degos L., Kieffer N., Lee H., Caen J. P. Monoclonal antibody to human platelet glycoprotein I. II. Effects on human platelet function. Br J Haematol. 1981 Dec;49(4):511–519. doi: 10.1111/j.1365-2141.1981.tb07259.x. [DOI] [PubMed] [Google Scholar]
  26. Ruggeri Z. M., Zimmerman T. S. Variant von Willebrand's disease: characterization of two subtypes by analysis of multimeric composition of factor VIII/von Willebrand factor in plasma and platelets. J Clin Invest. 1980 Jun;65(6):1318–1325. doi: 10.1172/JCI109795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sage H. Collagens of basement membranes. J Invest Dermatol. 1982 Jul;79 (Suppl 1):51s–59s. doi: 10.1111/1523-1747.ep12545773. [DOI] [PubMed] [Google Scholar]
  28. Sakariassen K. S., Bolhuis P. A., Sixma J. J. Human blood platelet adhesion to artery subendothelium is mediated by factor VIII-Von Willebrand factor bound to the subendothelium. Nature. 1979 Jun 14;279(5714):636–638. doi: 10.1038/279636a0. [DOI] [PubMed] [Google Scholar]
  29. Scott D. M., Griffin B., Pepper D. S., Barnes M. J. The binding of purified factor VIII/von Willebrand factor to collagens of differing type and form. Thromb Res. 1981 Dec 1;24(5-6):467–472. doi: 10.1016/0049-3848(81)90080-3. [DOI] [PubMed] [Google Scholar]
  30. Soberano M. E., Schoellmann G. Specificity of bacterial collagenase: studies with peptides newly synthesized using the solid-phase method. Biochim Biophys Acta. 1972 Jun 22;271(1):133–144. doi: 10.1016/0005-2795(72)90141-9. [DOI] [PubMed] [Google Scholar]
  31. Vaheri A., Kurkinen M., Lehto V. P., Linder E., Timpl R. Codistribution of pericellular matrix proteins in cultured fibroblasts and loss in transformation: fibronectin and procollagen. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4944–4948. doi: 10.1073/pnas.75.10.4944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wagner D. D., Olmsted J. B., Marder V. J. Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells. J Cell Biol. 1982 Oct;95(1):355–360. doi: 10.1083/jcb.95.1.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yaar M., Foidart J. M., Brown K. S., Rennard S. I., Martin G. R., Liotta L. The Goodpasture-like syndrome in mice induced by intravenous injections of anti-type IV collagen and anti-laminin antibody. Am J Pathol. 1982 Apr;107(1):79–91. [PMC free article] [PubMed] [Google Scholar]
  34. Zucker M. B., Broekman M. J., Kaplan K. L. Factor VIII-related antigen in human blood platelets: localization and release by thrombin and collagen. J Lab Clin Med. 1979 Nov;94(5):675–682. [PubMed] [Google Scholar]
  35. Zuckerman K. S., Wicha M. S. Extracellular matrix production by the adherent cells of long-term murine bone marrow cultures. Blood. 1983 Mar;61(3):540–547. [PubMed] [Google Scholar]