Biochemical identification of Citrobacter species defined by DNA hybridization and description of Citrobacter gillenii sp. nov. (formerly Citrobacter genomospecies 10) and Citrobacter murliniae sp. nov. (formerly Citrobacter genomospecies 11) - PubMed
Biochemical identification of Citrobacter species defined by DNA hybridization and description of Citrobacter gillenii sp. nov. (formerly Citrobacter genomospecies 10) and Citrobacter murliniae sp. nov. (formerly Citrobacter genomospecies 11)
D J Brenner et al. J Clin Microbiol. 1999 Aug.
Abstract
Recent work describing six named species and two unnamed genomospecies within Citrobacter has enlarged the genus to 11 species. DNA relatedness and phenotypic tests were used to determine how well these species can be identified. One hundred thirty-six strains were identified to species level by DNA relatedness and then identified phenotypically in a blinded fashion. By using conventional tests, 119 of the 136 strains (88%) were correctly identified to species level. Three additional strains (2%) were identified as citrobacteria but were not identified to species level, and 14 strains (10%) were misidentified as other Citrobacter species. Carbon source utilization tests were used to identify 86 of the strains. Eighty-four strains (98%) were correctly identified, and two strains (2%) were misidentified as other Citrobacter species. Additional strains of Citrobacter genomospecies 10 and Citrobacter genomospecies 11 were identified, allowing these species to be formally named as Citrobacter gillenii sp. nov. and Citrobacter murliniae sp. nov., respectively.
Similar articles
-
Brenner DJ, Grimont PA, Steigerwalt AG, Fanning GR, Ageron E, Riddle CF. Brenner DJ, et al. Int J Syst Bacteriol. 1993 Oct;43(4):645-58. doi: 10.1099/00207713-43-4-645. Int J Syst Bacteriol. 1993. PMID: 8240948
-
Gardan L, Shafik H, Belouin S, Broch R, Grimont F, Grimont PA. Gardan L, et al. Int J Syst Bacteriol. 1999 Apr;49 Pt 2:469-78. doi: 10.1099/00207713-49-2-469. Int J Syst Bacteriol. 1999. PMID: 10319466
-
O'Hara CM, Brenner FW, Steigerwalt AG, Hill BC, Holmes B, Grimont PA, Hawkey PM, Penner JL, Miller JM, Brenner DJ. O'Hara CM, et al. Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1869-1875. doi: 10.1099/00207713-50-5-1869. Int J Syst Evol Microbiol. 2000. PMID: 11034498
-
Genetic and biochemical characterization of Citrobacter rodentium sp. nov.
Schauer DB, Zabel BA, Pedraza IF, O'Hara CM, Steigerwalt AG, Brenner DJ. Schauer DB, et al. J Clin Microbiol. 1995 Aug;33(8):2064-8. doi: 10.1128/jcm.33.8.2064-2068.1995. J Clin Microbiol. 1995. PMID: 7559949 Free PMC article.
Cited by
-
Citrobacter bitternis sp. nov. isolated from bitterns.
Ko KS, Choi JY, Kim J, Park MK. Ko KS, et al. Curr Microbiol. 2015 Jun;70(6):894-7. doi: 10.1007/s00284-015-0810-1. Epub 2015 Apr 2. Curr Microbiol. 2015. PMID: 25833091
-
Draft Genome Sequence of Citrobacter gillenii MBT-C3, Isolated from Lamb's Lettuce.
Cho GS, Stein M, Bockelmann W, Neve H, Brinks E, Franz CMAP. Cho GS, et al. Microbiol Resour Announc. 2018 Oct 18;7(15):e01177-18. doi: 10.1128/MRA.01177-18. eCollection 2018 Oct. Microbiol Resour Announc. 2018. PMID: 30533725 Free PMC article.
-
Shan S, Cheng W, Li Y, Zhang M, Liu Z, Wang Y, Wei X, Fu Z, Wu S, Du D, Guo Z. Shan S, et al. BMC Genomics. 2022 Dec 19;23(1):838. doi: 10.1186/s12864-022-09069-4. BMC Genomics. 2022. PMID: 36536293 Free PMC article.
-
Complete genome sequence of Citrobacter werkmanii strain BF-6 isolated from industrial putrefaction.
Zhou G, Peng H, Wang YS, Huang XM, Xie XB, Shi QS. Zhou G, et al. BMC Genomics. 2017 Oct 10;18(1):765. doi: 10.1186/s12864-017-4157-9. BMC Genomics. 2017. PMID: 29017450 Free PMC article.
-
Venkidusamy K, Hari AR, Megharaj M. Venkidusamy K, et al. Front Microbiol. 2018 Mar 12;9:349. doi: 10.3389/fmicb.2018.00349. eCollection 2018. Front Microbiol. 2018. PMID: 29593662 Free PMC article.
References
-
- Bascomb S, Abbott S L, Bobolis J D, Bruckner D A, Connell S J, Cullen S K, Daugherty M, Glenn D, Janda J M, Lentsch S J, Lindquist D, Mayhew P B, Nothaft D M, Skinner J R, Williams G B, Wong J, Zimmer B L. Multicenter evaluation of the MicroScan rapid gram-negative identification type 3 panel. J Clin Microbiol. 1997;35:2531–2536. - PMC - PubMed
-
- Brenner D J, Grimont P A D, Steigerwalt A G, Fanning G R, Ageron E, Riddle C F. Classification of citrobacteria by DNA hybridization: designation of Citrobacter farmeri sp. nov., Citrobacter youngae sp. nov., Citrobacter braakii sp. nov., Citrobacter werkmanii sp. nov., Citrobacter sedlakii sp. nov., and three unnamed Citrobacter genomospecies. Int J Syst Bacteriol. 1993;43:645–658. - PubMed
-
- Brenner D J, McWhorter A, Kai A, Steigerwalt A G, Farmer J J., III Enterobacter asburiae: a new species found in human clinical specimens, and reassignment of Erwinia dissolvens and Erwinia nimipressuralis to the genus Enterobacter as Enterobacter dissolvens comb. nov. and Enterobacter nimipressuralis comb. nov. J Clin Microbiol. 1986;23:1114–1120. - PMC - PubMed
-
- Ewing W H. Edwards and Ewing’s identification of Enterobacteriaceae. 4th ed. New York, N.Y: Elsevier; 1986. pp. 346–347.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources