An adenoviral vector expressing the glucose transporter protects cultured striatal neurons from 3-nitropropionic acid - PubMed

An adenoviral vector expressing the glucose transporter protects cultured striatal neurons from 3-nitropropionic acid

S L Fink et al. Brain Res. 2000.

Free article

Abstract

Considerable interest has focused on the possibility of using gene transfer techniques to introduce protective genes into neurons around the time of necrotic insults. We have previously used herpes simplex virus amplicon vectors to overexpress the rat brain glucose transporter, Glut-1 (GT), and have shown it to protect against a variety of necrotic insults both in vitro and in vivo, as well as to buffer neurons from the steps thought to mediate necrotic injury. It is critical to show the specificity of the effects of any such transgene overexpression, in order to show that protection arises from the transgene delivered, rather than from the vector delivery system itself. As such, we tested the protective potential of GT overexpression driven, in this case, by an adenoviral vector, against a novel insult, namely exposure of primary striatal cultures to the metabolic poison, 3-nitropropionic acid (3NP). We observed that GT overexpression buffered neurons from neurotoxicity induced by 3NP.

Similar articles

• Defective herpes simplex virus vectors expressing the rat brain glucose transporter protect cultured neurons from necrotic insults.

  Ho DY, Saydam TC, Fink SL, Lawrence MS, Sapolsky RM. Ho DY, et al. J Neurochem. 1995 Aug;65(2):842-50. doi: 10.1046/j.1471-4159.1995.65020842.x. J Neurochem. 1995. PMID: 7616244

• Neuroprotective effects of an adenoviral vector expressing the glucose transporter: a detailed description of the mediating cellular events.

  Gupta A, Ho DY, Brooke S, Franklin L, Roy M, McLaughlin J, Fink SL, Sapolsky RM. Gupta A, et al. Brain Res. 2001 Jul 20;908(1):49-57. doi: 10.1016/s0006-8993(01)02572-0. Brain Res. 2001. PMID: 11457430

• Cyclosporin A protects striatal neurons in vitro and in vivo from 3-nitropropionic acid toxicity.

  Leventhal L, Sortwell CE, Hanbury R, Collier TJ, Kordower JH, Palfi S. Leventhal L, et al. J Comp Neurol. 2000 Oct 2;425(4):471-8. doi: 10.1002/1096-9861(20001002)425:4<471::aid-cne1>3.0.co;2-u. J Comp Neurol. 2000. PMID: 10975874

• Overexpression of the glucose transporter gene with a herpes simplex viral vector protects striatal neurons against stroke.

  Lawrence MS, Sun GH, Kunis DM, Saydam TC, Dash R, Ho DY, Sapolsky RM, Steinberg GK. Lawrence MS, et al. J Cereb Blood Flow Metab. 1996 Mar;16(2):181-5. doi: 10.1097/00004647-199603000-00001. J Cereb Blood Flow Metab. 1996. PMID: 8594048

• Astrocytes from cortex and striatum show differential responses to mitochondrial toxin and BDNF: implications for protection of striatal neurons expressing mutant huntingtin.

  Saba J, López Couselo F, Turati J, Carniglia L, Durand D, de Laurentiis A, Lasaga M, Caruso C. Saba J, et al. J Neuroinflammation. 2020 Oct 6;17(1):290. doi: 10.1186/s12974-020-01965-4. J Neuroinflammation. 2020. PMID: 33023623 Free PMC article.

Cited by

• Hypoxic preconditioning protects against ischemic brain injury.

  Sharp FR, Ran R, Lu A, Tang Y, Strauss KI, Glass T, Ardizzone T, Bernaudin M. Sharp FR, et al. NeuroRx. 2004 Jan;1(1):26-35. doi: 10.1602/neurorx.1.1.26. NeuroRx. 2004. PMID: 15717005 Free PMC article. Review.

• Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury.

  Li J, Xu X, Zhang S, Zheng M, Wu Z, Weng Y, Ouyang L, Yu J, Li F. Li J, et al. Neural Regen Res. 2012 Jun 15;7(17):1338-42. doi: 10.3969/j.issn.1673-5374.2012.17.009. Neural Regen Res. 2012. PMID: 25657665 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal