Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla - PubMed

Affiliations

• PMID: 14980488
• DOI: 10.1016/j.virol.2003.10.033

Free article

Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla

C P D Brussaard et al. Virology. 2004.

Free article

Abstract

We report the isolation of the first double-stranded (ds) RNA virus in the family Reoviridae that infects a protist (microalga Micromonas pusilla, Prasinophyceae). The dsRNA genome was composed of 11 segments ranging between 0.8 and 5.8 kb, with a total size of approximately 25.5 kb. The virus (MpRNAV-01B) could not be assigned to the genus level because host type, genome size, and number of segments smaller than 2 kb did not correspond to either of the two existing 11-segmented dsRNA genera Rotavirus and Aquareovirus. MpRNAV-01B has a particle size of 65-80 nm, a narrow host range, a latent period of 36 h, and contains five major proteins (120, 95, 67, 53, and 32 kDa). MpRNAV-01B was stable to freeze-thawing, resistant to chloroform, ether, nonionic detergents, chelating and reducing agents. The virus was inactivated at temperatures above 35 degrees C and by ionic detergent, ethanol, acetone, and acidic conditions (pH 2-5).

Similar articles

• Isolation and characterization of two viruses with large genome size infecting Chrysochromulina ericina (Prymnesiophyceae) and Pyramimonas orientalis (Prasinophyceae).

  Sandaa RA, Heldal M, Castberg T, Thyrhaug R, Bratbak G. Sandaa RA, et al. Virology. 2001 Nov 25;290(2):272-80. doi: 10.1006/viro.2001.1161. Virology. 2001. PMID: 11883191

• Complete nucleotide sequences and genome characterization of a novel double-stranded RNA virus infecting Rosa multiflora.

  Salem NM, Golino DA, Falk BW, Rowhani A. Salem NM, et al. Arch Virol. 2008;153(3):455-62. doi: 10.1007/s00705-007-0008-3. Epub 2008 Jan 3. Arch Virol. 2008. PMID: 18172568

• Complete nucleotide sequence and genome organization of a dsRNA partitivirus infecting Pleurotus ostreatus.

  Lim WS, Jeong JH, Jeong RD, Yoo YB, Yie SW, Kim KH. Lim WS, et al. Virus Res. 2005 Mar;108(1-2):111-9. doi: 10.1016/j.virusres.2004.08.017. Virus Res. 2005. PMID: 15681061

• Nonstructural proteins involved in genome packaging and replication of rotaviruses and other members of the Reoviridae.

  Taraporewala ZF, Patton JT. Taraporewala ZF, et al. Virus Res. 2004 Apr;101(1):57-66. doi: 10.1016/j.virusres.2003.12.006. Virus Res. 2004. PMID: 15010217 Review.

• Emerging themes in rotavirus cell entry, genome organization, transcription and replication.

  Jayaram H, Estes MK, Prasad BV. Jayaram H, et al. Virus Res. 2004 Apr;101(1):67-81. doi: 10.1016/j.virusres.2003.12.007. Virus Res. 2004. PMID: 15010218 Review.

Cited by

• Metagenomics reshapes the concepts of RNA virus evolution by revealing extensive horizontal virus transfer.

  Dolja VV, Koonin EV. Dolja VV, et al. Virus Res. 2018 Jan 15;244:36-52. doi: 10.1016/j.virusres.2017.10.020. Epub 2017 Nov 8. Virus Res. 2018. PMID: 29103997 Free PMC article. Review.

• The Phycodnaviridae: the story of how tiny giants rule the world.

  Wilson WH, Van Etten JL, Allen MJ. Wilson WH, et al. Curr Top Microbiol Immunol. 2009;328:1-42. doi: 10.1007/978-3-540-68618-7_1. Curr Top Microbiol Immunol. 2009. PMID: 19216434 Free PMC article. Review.

• Smaller fleas: viruses of microorganisms.

  Hyman P, Abedon ST. Hyman P, et al. Scientifica (Cairo). 2012;2012:734023. doi: 10.6064/2012/734023. Epub 2012 Jun 26. Scientifica (Cairo). 2012. PMID: 24278736 Free PMC article. Review.

• Tick-Borne Viruses.

  Shi J, Hu Z, Deng F, Shen S. Shi J, et al. Virol Sin. 2018 Feb;33(1):21-43. doi: 10.1007/s12250-018-0019-0. Epub 2018 Mar 13. Virol Sin. 2018. PMID: 29536246 Free PMC article. Review.

• Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes.

  Mazur-Marzec H, Andersson AF, Błaszczyk A, Dąbek P, Górecka E, Grabski M, Jankowska K, Jurczak-Kurek A, Kaczorowska AK, Kaczorowski T, Karlson B, Kataržytė M, Kobos J, Kotlarska E, Krawczyk B, Łuczkiewicz A, Piwosz K, Rybak B, Rychert K, Sjöqvist C, Surosz W, Szymczycha B, Toruńska-Sitarz A, Węgrzyn G, Witkowski A, Węgrzyn A. Mazur-Marzec H, et al. FEMS Microbiol Rev. 2024 Sep 18;48(5):fuae024. doi: 10.1093/femsre/fuae024. FEMS Microbiol Rev. 2024. PMID: 39366767 Free PMC article. Review.

Publication types

MeSH terms

Substances