pubmed.ncbi.nlm.nih.gov

Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces - PubMed

Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces

G L Wong et al. Proc Natl Acad Sci U S A. 1975 Aug.

Abstract

Six populations of bone cells (populations 1-6) were obtained by sequential digestion of mouse calvaria with collagenase and trypsin. After release from the tissue, each cell population was cultured for seven days. Parathormone, but not calcitonin, elicited an increase in intracellular cyclic AMP in the cells of populations 4, 5, and 6. In contrast, both hormones elicited increases in cyclic AMP in populations 2 and 3 but had no effect on population 1. When the cells of population 2 were exposed to a Falcontissue culture polystyrene surface for periods of time up to 5 min, many cells adhered. The nonadhering cell population contained a lesser proportion of cells responsive to calcitonin, whereas the adhering population contained a greater proportion responsive to this hormone. Conversely, when the cells of population 2 were exposed to an acid-treated nylon surface, the nonadhering cells contained a larger proportion of those responsive to calcitonin and a smaller proportion responsive to parathormone. When those cells that were enriched for calcitonin responsiveness were examined, we found an increased proportion that exhibited an asymmetric bipolar morphology. These differed from large amorphous, often binucleate, cells which predominated in those populations that responded exclusively to parathormone. These results establish that bone contains at least two types of target cells--one that responds to parathormone but not calcitonin, the other that responds predominantly to calcitonin.

PubMed Disclaimer

Cited by

Targeted disruption of ephrin B1 in cells of myeloid lineage increases osteoclast differentiation and bone resorption in mice.
Cheng S, Zhao SL, Nelson B, Kesavan C, Qin X, Wergedal J, Mohan S, Xing W. Cheng S, et al. PLoS One. 2012;7(3):e32887. doi: 10.1371/journal.pone.0032887. Epub 2012 Mar 5. PLoS One. 2012. PMID: 22403721 Free PMC article.
Biological characterization of interleukin-1-like cytokine produced by cultured bone cells from newborn mouse calvaria.
Hanazawa S, Amano S, Nakada K, Ohmori Y, Miyoshi T, Hirose K, Kitano S. Hanazawa S, et al. Calcif Tissue Int. 1987 Jul;41(1):31-7. doi: 10.1007/BF02555128. Calcif Tissue Int. 1987. PMID: 3113699
Involvement of integrins alpha(3)beta(1) and alpha(5)beta(1) and glycoprotein IIb in megakaryocyte-induced osteoblast proliferation.
Lemieux JM, Horowitz MC, Kacena MA. Lemieux JM, et al. J Cell Biochem. 2010 Apr 1;109(5):927-32. doi: 10.1002/jcb.22468. J Cell Biochem. 2010. PMID: 20052668 Free PMC article.
Keratocan is expressed by osteoblasts and can modulate osteogenic differentiation.
Igwe JC, Gao Q, Kizivat T, Kao WW, Kalajzic I. Igwe JC, et al. Connect Tissue Res. 2011 Oct;52(5):401-7. doi: 10.3109/03008207.2010.546536. Epub 2011 Mar 15. Connect Tissue Res. 2011. PMID: 21405980 Free PMC article.
Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin.
Vukicevic S, Luyten FP, Reddi AH. Vukicevic S, et al. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8793-7. doi: 10.1073/pnas.86.22.8793. Proc Natl Acad Sci U S A. 1989. PMID: 2554330 Free PMC article.

References

1. Science. 1964 Dec 11;146(3650):1476-7 - PubMed
1. Endocrinology. 1975 Jan;96(1):70-6 - PubMed
1. J Biol Chem. 1975 Feb 25;250(4):1354-60 - PubMed
1. Proc Natl Acad Sci U S A. 1975 Feb;72(2):474-8 - PubMed
1. Endocrinology. 1973 Apr;92(4):1282-5 - PubMed

Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces - PubMed