Xerophilic aflatoxigenic black tea fungi and their inhibition by Elettaria cardamomum and Syzygium aromaticum extracts - PubMed
Xerophilic aflatoxigenic black tea fungi and their inhibition by Elettaria cardamomum and Syzygium aromaticum extracts
Saleh Al-Sohaibani et al. Saudi J Biol Sci. 2011 Oct.
Abstract
Black tea is consumed worldwide and is believed to play a role in cancer prevention. Xerophilic aflatoxigenic fungi are highly hazardous contaminants of tea since they are associated with tea quality impairment and human health risk. The present study reports isolation of such xerophilic and aflatoxigenic fungi associated with marketed tea. Twenty different tea samples collected from the local markets of Tamilnadu, India were investigated for fungal contamination. The results indicated contamination by 0.38% Aspergillus flavus. Other common contaminant fungi including Penicillium spp. (0.30%), Pacelomyces spp. (0.14%), and Mucor spp. (0.19%) were also isolated. Amongst the fungi isolated Aspergillus niger ML01 and A. flavus ML02 were found to be xerophilic aflatoxigenic mycoflora. Phylogenetic analysis based on 28S rRNA revealed their close ancestry. The chloroform and acetone extracts of spices Elettaria cardamomum and Syzygium aromaticum exhibited antifungal inhibitory activity on growth and toxin elaboration of both these xerophilic tea contaminants A. niger ML01 and A. flavus ML02. The results advocate the use of these spices plant or their extracts as novel antimicrobials which may add preservation and flavour in marketed tea.
Keywords: Aflatoxin; Black tea; Elettaria cardamomum; Syzygium aromaticum; Xerophile.
Figures
The phylogentic tree of Aspergillus niger ML01 obtained by neighbour-joining method from 28S rDNA sequences.
The cladogram of phylogentic tree for Aspergillus flavus ML02 obtained by neighbour-joining method from 28S rDNA sequences.
Similar articles
-
Fungi associated with black tea and tea quality in the Sultanate of Oman.
Elshafie AE, Al-Lawatia T, Al-Bahry S. Elshafie AE, et al. Mycopathologia. 1999;145(2):89-93. doi: 10.1023/a:1007034729467. Mycopathologia. 1999. PMID: 10598069
-
Aflatoxigenic and ochratoxigenic fungi and their mycotoxins in spices marketed in Brazil.
Garcia MV, Mallmann CA, Copetti MV. Garcia MV, et al. Food Res Int. 2018 Apr;106:136-140. doi: 10.1016/j.foodres.2017.12.061. Epub 2017 Dec 26. Food Res Int. 2018. PMID: 29579911
-
Fungi in spices and mycotoxigenic potential of some Aspergilli isolated.
Garcia MV, Parussolo G, Moro CB, Bernardi AO, Copetti MV. Garcia MV, et al. Food Microbiol. 2018 Aug;73:93-98. doi: 10.1016/j.fm.2018.01.013. Epub 2018 Jan 16. Food Microbiol. 2018. PMID: 29526231
-
Fungi and aflatoxins associated with spices in the Sultanate of Oman.
Elshafie AE, Al-Rashdi TA, Al-Bahry SN, Bakheit CS. Elshafie AE, et al. Mycopathologia. 2002;155(3):155-60. doi: 10.1023/a:1020427527963. Mycopathologia. 2002. PMID: 12617502
-
Tian F, Lee SY, Woo SY, Chun HS. Tian F, et al. Front Microbiol. 2020 Mar 17;11:419. doi: 10.3389/fmicb.2020.00419. eCollection 2020. Front Microbiol. 2020. PMID: 32256475 Free PMC article. Review.
Cited by
-
Biotechnological potential of salt tolerant and xerophilic species of Aspergillus.
Pócsi I, Dijksterhuis J, Houbraken J, de Vries RP. Pócsi I, et al. Appl Microbiol Biotechnol. 2024 Nov 19;108(1):521. doi: 10.1007/s00253-024-13338-5. Appl Microbiol Biotechnol. 2024. PMID: 39560743 Free PMC article. Review.
References
-
- 1) Alexopoules C.J., Mims C.W., Blackwell M. John Wiley and Sons, Inc.; New York: 1996. Introductory Mycology.
-
- AOAC . 17th ed. AOAC International; Maryland, USA: 2000. Official Methods of Analysis.
-
- Banerjee S., Panda C.K., Das S. Clove (Syzygium aromaticum L.), a potential chemopreventive agent for lung cancer. Carcinogenesis. 2006;27:1645. - PubMed
-
- Beuchat L.R., Montville T.J. ASM Publications; Washington, DC: 1989. Food microbiology Fundamentals and Frontiers. pp. 520–556.
-
- Billing J., Sherman P.W. Antimicrobial functions of spices: why some like it hot. Quart. Rev. Biol. 1998;73:3–49. - PubMed
LinkOut - more resources
Full Text Sources
Miscellaneous