pubmed.ncbi.nlm.nih.gov

Differentially Expressed Circular RNA Profile in an Intracranial Aneurysm Group Compared with a Healthy Control Group - PubMed

  • ️Fri Jan 01 2021

Differentially Expressed Circular RNA Profile in an Intracranial Aneurysm Group Compared with a Healthy Control Group

Yonggang Ma et al. Dis Markers. 2021.

Abstract

Objective: Intracranial aneurysm (IA) is a fatal disease owing to vascular rupture and subarachnoid hemorrhage. Much attention has been given to circular RNAs (circRNAs) because they may be potential biomarkers for many diseases, but their mechanism in the formation of IA remains unknown.

Methods: circRNA expression profile analysis of blood samples was conducted between patients with IA and controls. Overall, 235 differentially expressed circRNAs were confirmed between IA patients and the control group. The reliability of the microarray results was demonstrated by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: Of 235 differentially expressed genes, 150 were upregulated, while the other 85 were downregulated. Five miRNAs matched to every differential expression of circRNAs, and related MREs were predicted. We performed gene ontology (GO) analysis to identify the functions of their targeted genes, with the terms "Homophilic cell adhesion via plasma membrane adhesion molecules" and "Positive regulation of cellular process" showing the highest fold enrichment.

Conclusions: This study demonstrated the role of circRNA expression profiling in the formation of IA and revealed that the mTOR pathway can be a latent therapeutic strategy for IA.

Copyright © 2021 Yonggang Ma et al.

PubMed Disclaimer

Conflict of interest statement

The authors have no financial conflicts of interest.

Figures

Figure 1
Figure 1

circRNA expression profile comparison among three groups. (a) Hierarchical clustering demonstrates a difference in circRNA expression profiling between the three groups. (b) Volcano plot shows differential circRNA expression between the IA patients and control groups, and the red points represent the circRNAs with log 2.0-fold changes (upregulated and downregulated) with statistical significance (P < 0.05). (c) Scattered plot shows the circRNA expression variation between the IA patients and control groups. (d) Box plot shows the distribution of circRNA expression patterns of three groups. IA: aneurysm.

Figure 2
Figure 2

qRT-PCR validation of the differentially expressed circRNAs.

Figure 3
Figure 3

GO and KEGG pathway analyses of the differential expression circRNAs. (a), Top ten GO biological processes. (b), KEGG pathways of differential expression circRNAs. GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes.

Figure 4
Figure 4

Target genes in the mTOR signaling pathway. mTOR: mammalian target of rapamycin.

Similar articles

Cited by

References

    1. Vlak M. H., Algra A., Brandenburg R., Rinkel G. J. Prevalence of unruptured intracranial aneurysms, with emphasis on sex, age, comorbidity, country, and time period: a systematic review and meta-analysis. Lancet Neurology. 2011;10(7):626–636. doi: 10.1016/S1474-4422(11)70109-0. - DOI - PubMed
    1. Chalouhi N., Ali M. S., Jabbour P. M., et al. Biology of intracranial aneurysms: role of inflammation. Journal of Cerebral Blood Flow and Metabolism. 2012;32(9):1659–1676. doi: 10.1038/jcbfm.2012.84. - DOI - PMC - PubMed
    1. Aoki T., Kataoka H., Ishibashi R., Nozaki K., Egashira K., Hashimoto N. Impact of monocyte chemoattractant protein-1 deficiency on cerebral aneurysm formation. Stroke. 2009;40(3):942–951. doi: 10.1161/STROKEAHA.108.532556. - DOI - PubMed
    1. Koffie R. M., Stapleton C. J., Torok C. M., Yoo A. J., Leslie-Mazwi T. M., Codd P. J. Rapid growth of an infectious intracranial aneurysm with catastrophic intracranial hemorrhage. Journal of Clinical Neuroscience. 2015;22(3):603–605. doi: 10.1016/j.jocn.2014.09.007. - DOI - PubMed
    1. Feigin V., Parag V., Lawes C. M., et al. Smoking and elevated blood pressure are the most important risk factors for subarachnoid hemorrhage in the asia-pacific region: an overview of 26 cohorts involving 306, 620 participants. Stroke. 2005;36(7):1360–1365. doi: 10.1161/01.STR.0000170710.95689.41. - DOI - PubMed

MeSH terms

Substances